Myocardial protection with and without leukocyte depletion: A comparative study on the oxidative stress

M. G. Pala, G. Paolini, R. Paroni, E. De Vecchi, C. Gallorini, P. L. Stefano, G. Di Credico, M. Zuccari, L. Galli, V. Agape, G. Marino, A. Grossi

Research output: Contribution to journalArticlepeer-review

Abstract

We tested the hypothesis that controlled reperfusion with leukocyte-depleted blood could improve myocardial protection by reducing theoxidative stress in patients undergoing myocardial revascularization.Thirty-four patients receiving antegrade/retrograde blood cardioplegia weredivided into: group A: 11 patients with ejection fractions (EF) less than35%, treated with leukocyte-depleted controlled blood reperfusion, group B:11 patients with EF less than 35% in whom no leukocyte depletion wasperformed, group C: 6 patients with EF more than 45% treated as group A andgroup D: 6 patients with EF more than 45% without leukocyte depletion. Toasses the oxidative stress, we evaluated total, total oxidized (GSSX), andreduced glutathione (GSH) in coronary sinus plasma, immediately beforecross-clamping the aorta (T0), and at 0 (T1), 15 (T2) and 30 (T3) min afterunclamping it. In groups A and B a significant shift towards oxidation ofredox status of glutathione (GSH/GSSX) at T1 vs T0 was observed.Glutathione redox ratio remained low in group B while in group A itreturned to the basal value at T2 with a significant difference from groupB at T2 and T3. No differences were observed between groups C and D. Inconclusion, our data show that leukocyte-depleted reperfusion can afford abetter myocardial protection in patients with left ventricular dysfunction, while it seems unnecessary in patients with normal EF.

Original languageEnglish
Pages (from-to)701-706
Number of pages6
JournalEuropean Journal of Cardio-thoracic Surgery
Volume9
Issue number12
DOIs
Publication statusPublished - 1995

Keywords

  • Glutathione
  • Myocardial reperfusion injury
  • Myocardial revascularization
  • Neutrophils
  • Oxidative stress

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Surgery
  • Pulmonary and Respiratory Medicine

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