Myofibrillar ATPase activity in skinned human skeletal muscle fibres: Fibre type and temperature dependence

G. J M Stienen, J. L. Kiers, R. Bottinelli, C. Reggiani

Research output: Contribution to journalArticle

Abstract

1. Myofibrillar ATP consumption and isometric tension (P0) were determined in chemically skinned skeletal muscle fibres from human rectus abdominis and vastus lateralis muscle. Fibres were classified in four groups (I, II A, IIB, IIA/B or mixed) based on myosin heavy chain composition. 2. ATP consumption (± S.E.M.) at 20°C varied from 0·41 ± 0·06 mmol l-1 s-1 in type IIB fibres (n = 5) to 0·10 ± 0·01 mmol l-1 s-1 in type I fibres (n = 13). 3. The ratio between ATPase activity and P0 (tension cost) differed significantly between fast type II and slow type I fibres. At 12°C tension cost was lower than the values found previously in corresponding fibre types in the rat. 4. The relative increase in ATPase activity for a 10°C temperature change (Q10), determined in the range from 12 to 30°C, was temperature independent and amounted to 2·60 ± 0·06. The increase in P0 with temperature was smaller and declined when the temperature increased. 5. From these measurements, estimates were obtained for the maximum rate of isometric ATP consumption and force development at muscle temperature in vivo (35°C).

Original languageEnglish
Pages (from-to)299-307
Number of pages9
JournalJournal of Physiology
Volume493
Issue number2
Publication statusPublished - Jun 1 1996

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Skeletal Muscle Fibers
Adenosine Triphosphatases
Temperature
Adenosine Triphosphate
Costs and Cost Analysis
Rectus Abdominis
Myosin Heavy Chains
Muscle Development
Quadriceps Muscle
Muscles

ASJC Scopus subject areas

  • Physiology

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Myofibrillar ATPase activity in skinned human skeletal muscle fibres : Fibre type and temperature dependence. / Stienen, G. J M; Kiers, J. L.; Bottinelli, R.; Reggiani, C.

In: Journal of Physiology, Vol. 493, No. 2, 01.06.1996, p. 299-307.

Research output: Contribution to journalArticle

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