Myogenic induction of adult and pluripotent stem cells using recombinant proteins

Ilaria Perini, Ilaria Elia, Antonio Lo Nigro, Flavio Ronzoni, Emanuele Berardi, Hanne Grosemans, So Ichiro Fukada, Maurilio Sampaolesi

Research output: Contribution to journalArticlepeer-review

Abstract

Abstract Met Activating Genetically Improved Chimeric Factor 1 (Magic-F1) is a human recombinant protein, derived from dimerization of the receptor-binding domain of hepatocyte growth factor. Previous experiments demonstrate that in transgenic mice, the skeletal muscle specific expression of Magic-F1 can induce a constitutive muscular hypertrophy, improving running performance and accelerating muscle regeneration after injury. In order to evaluate the therapeutic potential of Magic-F1, we tested its effect on multipotent and pluripotent stem cells. In murine mesoangioblasts (adult vessel-associated stem cells), the presence of Magic-F1 did not alter their osteogenic, adipogenic or smooth muscle differentiation ability. However, when analyzing their myogenic potential, mesoangioblasts expressing Magic-F1 differentiated spontaneously into myotubes. Finally, Magic-F1 inducible cassette was inserted into a murine embryonic stem cell line by homologous recombination. When embryonic stem cells were subjected to myogenic differentiation, the presence of Magic-F1 resulted in the upregulation of Pax3 and Pax7 that enhanced the myogenic commitment of transgenic pluripotent stem cells. Taken together our results candidate Magic-F1 as a potent myogenic stimulator, able to enhance muscular differentiation from both adult and pluripotent stem cells.

Original languageEnglish
Article number34230
Pages (from-to)755-761
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume464
Issue number3
DOIs
Publication statusPublished - Jun 27 2015

Keywords

  • Embryonic stem cells
  • Magic-F1
  • Mesoangioblast
  • Myogenic differentiation
  • Recombinant protein

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology
  • Medicine(all)

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