Abstract
Abstract Met Activating Genetically Improved Chimeric Factor 1 (Magic-F1) is a human recombinant protein, derived from dimerization of the receptor-binding domain of hepatocyte growth factor. Previous experiments demonstrate that in transgenic mice, the skeletal muscle specific expression of Magic-F1 can induce a constitutive muscular hypertrophy, improving running performance and accelerating muscle regeneration after injury. In order to evaluate the therapeutic potential of Magic-F1, we tested its effect on multipotent and pluripotent stem cells. In murine mesoangioblasts (adult vessel-associated stem cells), the presence of Magic-F1 did not alter their osteogenic, adipogenic or smooth muscle differentiation ability. However, when analyzing their myogenic potential, mesoangioblasts expressing Magic-F1 differentiated spontaneously into myotubes. Finally, Magic-F1 inducible cassette was inserted into a murine embryonic stem cell line by homologous recombination. When embryonic stem cells were subjected to myogenic differentiation, the presence of Magic-F1 resulted in the upregulation of Pax3 and Pax7 that enhanced the myogenic commitment of transgenic pluripotent stem cells. Taken together our results candidate Magic-F1 as a potent myogenic stimulator, able to enhance muscular differentiation from both adult and pluripotent stem cells.
| Original language | English |
|---|---|
| Article number | 34230 |
| Pages (from-to) | 755-761 |
| Number of pages | 7 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 464 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Jun 27 2015 |
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Keywords
- Embryonic stem cells
- Magic-F1
- Mesoangioblast
- Myogenic differentiation
- Recombinant protein
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Cell Biology
- Molecular Biology
- Medicine(all)
Cite this
Myogenic induction of adult and pluripotent stem cells using recombinant proteins. / Perini, Ilaria; Elia, Ilaria; Lo Nigro, Antonio; Ronzoni, Flavio; Berardi, Emanuele; Grosemans, Hanne; Fukada, So Ichiro; Sampaolesi, Maurilio.
In: Biochemical and Biophysical Research Communications, Vol. 464, No. 3, 34230, 27.06.2015, p. 755-761.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Myogenic induction of adult and pluripotent stem cells using recombinant proteins
AU - Perini, Ilaria
AU - Elia, Ilaria
AU - Lo Nigro, Antonio
AU - Ronzoni, Flavio
AU - Berardi, Emanuele
AU - Grosemans, Hanne
AU - Fukada, So Ichiro
AU - Sampaolesi, Maurilio
PY - 2015/6/27
Y1 - 2015/6/27
N2 - Abstract Met Activating Genetically Improved Chimeric Factor 1 (Magic-F1) is a human recombinant protein, derived from dimerization of the receptor-binding domain of hepatocyte growth factor. Previous experiments demonstrate that in transgenic mice, the skeletal muscle specific expression of Magic-F1 can induce a constitutive muscular hypertrophy, improving running performance and accelerating muscle regeneration after injury. In order to evaluate the therapeutic potential of Magic-F1, we tested its effect on multipotent and pluripotent stem cells. In murine mesoangioblasts (adult vessel-associated stem cells), the presence of Magic-F1 did not alter their osteogenic, adipogenic or smooth muscle differentiation ability. However, when analyzing their myogenic potential, mesoangioblasts expressing Magic-F1 differentiated spontaneously into myotubes. Finally, Magic-F1 inducible cassette was inserted into a murine embryonic stem cell line by homologous recombination. When embryonic stem cells were subjected to myogenic differentiation, the presence of Magic-F1 resulted in the upregulation of Pax3 and Pax7 that enhanced the myogenic commitment of transgenic pluripotent stem cells. Taken together our results candidate Magic-F1 as a potent myogenic stimulator, able to enhance muscular differentiation from both adult and pluripotent stem cells.
AB - Abstract Met Activating Genetically Improved Chimeric Factor 1 (Magic-F1) is a human recombinant protein, derived from dimerization of the receptor-binding domain of hepatocyte growth factor. Previous experiments demonstrate that in transgenic mice, the skeletal muscle specific expression of Magic-F1 can induce a constitutive muscular hypertrophy, improving running performance and accelerating muscle regeneration after injury. In order to evaluate the therapeutic potential of Magic-F1, we tested its effect on multipotent and pluripotent stem cells. In murine mesoangioblasts (adult vessel-associated stem cells), the presence of Magic-F1 did not alter their osteogenic, adipogenic or smooth muscle differentiation ability. However, when analyzing their myogenic potential, mesoangioblasts expressing Magic-F1 differentiated spontaneously into myotubes. Finally, Magic-F1 inducible cassette was inserted into a murine embryonic stem cell line by homologous recombination. When embryonic stem cells were subjected to myogenic differentiation, the presence of Magic-F1 resulted in the upregulation of Pax3 and Pax7 that enhanced the myogenic commitment of transgenic pluripotent stem cells. Taken together our results candidate Magic-F1 as a potent myogenic stimulator, able to enhance muscular differentiation from both adult and pluripotent stem cells.
KW - Embryonic stem cells
KW - Magic-F1
KW - Mesoangioblast
KW - Myogenic differentiation
KW - Recombinant protein
UR - http://www.scopus.com/inward/record.url?scp=84938747281&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84938747281&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2015.07.022
DO - 10.1016/j.bbrc.2015.07.022
M3 - Article
C2 - 26164231
AN - SCOPUS:84938747281
VL - 464
SP - 755
EP - 761
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 3
M1 - 34230
ER -