Myogenic potential of adipose-tissue-derived cells

Giuliana Di Rocco, Maria Grazia Iachininoto, Alessandra Tritarelli, Stefania Straino, Antonella Zacheo, Antonia Germani, Filippo Crea, Maurizio C. Capogrossi

Research output: Contribution to journalArticle

167 Citations (Scopus)

Abstract

Adipose-tissue-derived mesenchymal stem cells can be directed towards a myogenic phenotype in vitro by the addition of specific inductive media. However, the ability of these or other adipose-tissue-associated cells to respond to 'natural' myogenic cues such as a myogenic environment has never been investigated in detail. Here, we provide evidence that a restricted subpopulation of freshly harvested adipose-tissue-derived cells possesses an intrinsic myogenic potential and can spontaneously differentiate into skeletal muscle. Conversion of adipose-tissue-derived cells to a myogenic phenotype is enhanced by co-culture with primary myoblasts in the absence of cell contact and is maximal when the two cell types are co-cultured in the same plate. Conversely, in vitro expanded adipose-tissue-derived mesenchymal stem cells require direct contact with muscle cells to generate skeletal myotubes. Finally, we show that uncultured adipose-tissue-associated cells have a high regenerative capacity in vivo since they can be incorporated into muscle fibers following ischemia and can restore significantly dystrophin expression in mdx mice.

Original languageEnglish
Pages (from-to)2945-2952
Number of pages8
JournalJournal of Cell Science
Volume119
Issue number14
DOIs
Publication statusPublished - Jul 15 2006

Fingerprint

Adipose Tissue
Mesenchymal Stromal Cells
Inbred mdx Mouse
Phenotype
Dystrophin
Aptitude
Myoblasts
Skeletal Muscle Fibers
Coculture Techniques
Muscle Cells
Cues
Cultured Cells
Skeletal Muscle
Ischemia
Muscles
In Vitro Techniques

Keywords

  • Adipose tissue
  • Cell transplantation
  • Muscular dystrophy
  • Myogenic differentiation

ASJC Scopus subject areas

  • Cell Biology

Cite this

Di Rocco, G., Iachininoto, M. G., Tritarelli, A., Straino, S., Zacheo, A., Germani, A., ... Capogrossi, M. C. (2006). Myogenic potential of adipose-tissue-derived cells. Journal of Cell Science, 119(14), 2945-2952. https://doi.org/10.1242/jcs.03029

Myogenic potential of adipose-tissue-derived cells. / Di Rocco, Giuliana; Iachininoto, Maria Grazia; Tritarelli, Alessandra; Straino, Stefania; Zacheo, Antonella; Germani, Antonia; Crea, Filippo; Capogrossi, Maurizio C.

In: Journal of Cell Science, Vol. 119, No. 14, 15.07.2006, p. 2945-2952.

Research output: Contribution to journalArticle

Di Rocco, G, Iachininoto, MG, Tritarelli, A, Straino, S, Zacheo, A, Germani, A, Crea, F & Capogrossi, MC 2006, 'Myogenic potential of adipose-tissue-derived cells', Journal of Cell Science, vol. 119, no. 14, pp. 2945-2952. https://doi.org/10.1242/jcs.03029
Di Rocco G, Iachininoto MG, Tritarelli A, Straino S, Zacheo A, Germani A et al. Myogenic potential of adipose-tissue-derived cells. Journal of Cell Science. 2006 Jul 15;119(14):2945-2952. https://doi.org/10.1242/jcs.03029
Di Rocco, Giuliana ; Iachininoto, Maria Grazia ; Tritarelli, Alessandra ; Straino, Stefania ; Zacheo, Antonella ; Germani, Antonia ; Crea, Filippo ; Capogrossi, Maurizio C. / Myogenic potential of adipose-tissue-derived cells. In: Journal of Cell Science. 2006 ; Vol. 119, No. 14. pp. 2945-2952.
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