Myogenic regional responsiveness to cholinergic and vipergic stimulation in human colon

M. A. Maselli, P. Trisolini, C. Petitta, D. Lorusso, A. Cicenia, A. Scirocco, F. Pezzolla, C. Severi

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Background Differences in the actions of enteric neurotransmitters on colonic circular and longitudinal muscle layers have not been clearly determined, nor the possible existence of intrinsic myogenic phenotypes that might contribute to regional differences in human colon motor activity. The aim of this study was to analyze the direct pharmaco-mechanical coupling of carbachol (CCh) and vasoactive intestinal polypeptide (VIP) on human colonic smooth muscle strips and cells. Methods Circular and longitudinal muscle strips and cells were obtained from 15 human specimens of ascending and sigmoid colon. Both isometric tension on muscle strips and contraction and relaxation on cells were measured in response to increasing CCh and VIP concentrations. Key Results Circular muscle strips of ascending colon were more sensitive to the effect of CCh than that of sigmoid colon, EC50 values being, respectively, 4.15μmolL-1 and 8.47μmolL-1 (P50: 0.041 and 0.15μmolL-1, respectively, P50: 0.043 and 0.09μmolL-1, respectively, P50: 44.85 and 75.24nmolL-1, respectively, P

Original languageEnglish
JournalNeurogastroenterology and Motility
Volume24
Issue number9
DOIs
Publication statusPublished - Sep 2012

Fingerprint

Carbachol
Cholinergic Agents
Ascending Colon
Colon
Vasoactive Intestinal Peptide
Sigmoid Colon
Muscles
Muscle Relaxation
Muscle Contraction
Muscle Cells
Smooth Muscle Myocytes
Neurotransmitter Agents
Motor Activity
Phenotype

Keywords

  • Ascending and sigmoid colon
  • Carbachol
  • Human
  • Smooth muscle
  • Vasoactive intestinal polypeptide

ASJC Scopus subject areas

  • Endocrine and Autonomic Systems
  • Gastroenterology
  • Physiology

Cite this

Myogenic regional responsiveness to cholinergic and vipergic stimulation in human colon. / Maselli, M. A.; Trisolini, P.; Petitta, C.; Lorusso, D.; Cicenia, A.; Scirocco, A.; Pezzolla, F.; Severi, C.

In: Neurogastroenterology and Motility, Vol. 24, No. 9, 09.2012.

Research output: Contribution to journalArticle

Maselli, M. A. ; Trisolini, P. ; Petitta, C. ; Lorusso, D. ; Cicenia, A. ; Scirocco, A. ; Pezzolla, F. ; Severi, C. / Myogenic regional responsiveness to cholinergic and vipergic stimulation in human colon. In: Neurogastroenterology and Motility. 2012 ; Vol. 24, No. 9.
@article{7498a04340a3418daf19da21a937fa1d,
title = "Myogenic regional responsiveness to cholinergic and vipergic stimulation in human colon",
abstract = "Background Differences in the actions of enteric neurotransmitters on colonic circular and longitudinal muscle layers have not been clearly determined, nor the possible existence of intrinsic myogenic phenotypes that might contribute to regional differences in human colon motor activity. The aim of this study was to analyze the direct pharmaco-mechanical coupling of carbachol (CCh) and vasoactive intestinal polypeptide (VIP) on human colonic smooth muscle strips and cells. Methods Circular and longitudinal muscle strips and cells were obtained from 15 human specimens of ascending and sigmoid colon. Both isometric tension on muscle strips and contraction and relaxation on cells were measured in response to increasing CCh and VIP concentrations. Key Results Circular muscle strips of ascending colon were more sensitive to the effect of CCh than that of sigmoid colon, EC50 values being, respectively, 4.15μmolL-1 and 8.47μmolL-1 (P50: 0.041 and 0.15μmolL-1, respectively, P50: 0.043 and 0.09μmolL-1, respectively, P50: 44.85 and 75.24nmolL-1, respectively, P",
keywords = "Ascending and sigmoid colon, Carbachol, Human, Smooth muscle, Vasoactive intestinal polypeptide",
author = "Maselli, {M. A.} and P. Trisolini and C. Petitta and D. Lorusso and A. Cicenia and A. Scirocco and F. Pezzolla and C. Severi",
year = "2012",
month = "9",
doi = "10.1111/j.1365-2982.2012.01927.x",
language = "English",
volume = "24",
journal = "Neurogastroenterology and Motility",
issn = "1350-1925",
publisher = "Wiley-Blackwell",
number = "9",

}

TY - JOUR

T1 - Myogenic regional responsiveness to cholinergic and vipergic stimulation in human colon

AU - Maselli, M. A.

AU - Trisolini, P.

AU - Petitta, C.

AU - Lorusso, D.

AU - Cicenia, A.

AU - Scirocco, A.

AU - Pezzolla, F.

AU - Severi, C.

PY - 2012/9

Y1 - 2012/9

N2 - Background Differences in the actions of enteric neurotransmitters on colonic circular and longitudinal muscle layers have not been clearly determined, nor the possible existence of intrinsic myogenic phenotypes that might contribute to regional differences in human colon motor activity. The aim of this study was to analyze the direct pharmaco-mechanical coupling of carbachol (CCh) and vasoactive intestinal polypeptide (VIP) on human colonic smooth muscle strips and cells. Methods Circular and longitudinal muscle strips and cells were obtained from 15 human specimens of ascending and sigmoid colon. Both isometric tension on muscle strips and contraction and relaxation on cells were measured in response to increasing CCh and VIP concentrations. Key Results Circular muscle strips of ascending colon were more sensitive to the effect of CCh than that of sigmoid colon, EC50 values being, respectively, 4.15μmolL-1 and 8.47μmolL-1 (P50: 0.041 and 0.15μmolL-1, respectively, P50: 0.043 and 0.09μmolL-1, respectively, P50: 44.85 and 75.24nmolL-1, respectively, P

AB - Background Differences in the actions of enteric neurotransmitters on colonic circular and longitudinal muscle layers have not been clearly determined, nor the possible existence of intrinsic myogenic phenotypes that might contribute to regional differences in human colon motor activity. The aim of this study was to analyze the direct pharmaco-mechanical coupling of carbachol (CCh) and vasoactive intestinal polypeptide (VIP) on human colonic smooth muscle strips and cells. Methods Circular and longitudinal muscle strips and cells were obtained from 15 human specimens of ascending and sigmoid colon. Both isometric tension on muscle strips and contraction and relaxation on cells were measured in response to increasing CCh and VIP concentrations. Key Results Circular muscle strips of ascending colon were more sensitive to the effect of CCh than that of sigmoid colon, EC50 values being, respectively, 4.15μmolL-1 and 8.47μmolL-1 (P50: 0.041 and 0.15μmolL-1, respectively, P50: 0.043 and 0.09μmolL-1, respectively, P50: 44.85 and 75.24nmolL-1, respectively, P

KW - Ascending and sigmoid colon

KW - Carbachol

KW - Human

KW - Smooth muscle

KW - Vasoactive intestinal polypeptide

UR - http://www.scopus.com/inward/record.url?scp=84865404945&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84865404945&partnerID=8YFLogxK

U2 - 10.1111/j.1365-2982.2012.01927.x

DO - 10.1111/j.1365-2982.2012.01927.x

M3 - Article

VL - 24

JO - Neurogastroenterology and Motility

JF - Neurogastroenterology and Motility

SN - 1350-1925

IS - 9

ER -