Myosin and actin content of human skeletal muscle fibers following 35 days bed rest

E. Borina, M. A. Pellegrino, G. D'Antona, R. Bottinelli

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Biopsy samples were taken from the vastus lateralis muscle of seven male subjects pre- and post-35 days bed rest (BR). The myosin heavy chain (MHC) isoform distribution of the samples was determined by densitometry of MHC bands separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Individual muscle fibers were dissected from biopsy samples pre-BR (n=143) and post-BR (n=144). They were studied as regards cross-sectional area (CSA), myosin content by quantitative electrophoresis and myosin actin (M/A) ratio by densitometry of myosin and actin bands of individual muscle fibers. All fibers were typed according to their MHC isoform content determined by SDS-PAGE. A decrease in MHC-1 relative content and an increase in MHC-2X content of whole muscle samples were found, suggesting a slow to fast shift in muscle phenotype. Consistently, fiber type distribution was shifted toward type 2X and 2AX fibers. Muscle fiber atrophy occurred at variable extent among fiber types. Myosin concentration was significantly lower in type 1 and type 2A muscle fibers post-BR than pre-BR, whereas M/A ratio did not vary. The latter findings indicate a disproportionate loss of myosin compared with fiber CSA and a proportional loss of myosin and actin.

Original languageEnglish
Pages (from-to)65-73
Number of pages9
JournalScandinavian Journal of Medicine and Science in Sports
Volume20
Issue number1
DOIs
Publication statusPublished - Feb 2010

Fingerprint

Bed Rest
Skeletal Muscle Fibers
Myosins
Actins
Myosin Heavy Chains
Muscles
Densitometry
Sodium Dodecyl Sulfate
Polyacrylamide Gel Electrophoresis
Protein Isoforms
Slow-Twitch Muscle Fibers
Biopsy
Muscular Atrophy
Quadriceps Muscle
Electrophoresis
Phenotype

Keywords

  • Bed rest
  • Muscle atrophy
  • Myosin content
  • Skinned fibers

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

Cite this

Myosin and actin content of human skeletal muscle fibers following 35 days bed rest. / Borina, E.; Pellegrino, M. A.; D'Antona, G.; Bottinelli, R.

In: Scandinavian Journal of Medicine and Science in Sports, Vol. 20, No. 1, 02.2010, p. 65-73.

Research output: Contribution to journalArticle

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