n-3 PUFA supplementation, monocyte PCA expression and interleukin-6 production

Rosanna Abbate, Anna Maria Gori, Francesca Martini, Tamara Brunelli, Monica Filippini, Isa Francalanci, Rita Paniccia, Domenico Prisco, Gian Franco Gensini, Gian Gastone Neri Serneri

Research output: Contribution to journalArticle


n-3 polyunsaturated fatty acids (PUFA) can affect several monocyte functions and the biochemistry of blood cells, thus possibly influencing the initiation of thrombosis, inflammatory disease and atherosclerosis. In this study, we have investigated the effect of dietary supplementation with n-3 PUFA ethyl esters on procoagulant activity (PCA) and interleukin-6 (IL-6) production by human mononuclear cells. Nine healthy volunteers received 4 g/d of n-3 PUFA ethyl esters (4 x 1 g capsules with at least 85% eicosapentaenoic + docosahexaenoic acid ethyl esters) for 18 weeks. Before and at the end of the treatment, mononuclear cells were obtained from peripheral citrated blood by Ficoll-Hypaque density gradient centrifugation. Cellular suspensions (107 cells/ml) were incubated at 37°C for 4 h in the absence and presence of lipopolysaccharide (10 μg/ml); PCA was determined by one-stage clotting assay and IL-6 concentrations were assayed in supernatants by specific ELISA. After 18-week treatment, both unstimulated and stimulated monocyte PCA were significantly reduced by 66% and 63%, respectively (P <0.01). Similarly, a significant inhibitory effect by n-3 PUFA treatment on basal and LPS-stimulated IL-6 monocyte production was observed (50% and 46%, respectively, P <0.05). These data indicate that 18-week n-3 PUFA supplementation may influence monocyte activities, which play a specific role in atherosclerosis and its thrombotic complications.

Original languageEnglish
Pages (from-to)439-444
Number of pages6
JournalProstaglandins, Leukotrienes and Essential Fatty Acids
Issue number6
Publication statusPublished - Jun 1996

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Endocrinology, Diabetes and Metabolism

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    Abbate, R., Gori, A. M., Martini, F., Brunelli, T., Filippini, M., Francalanci, I., Paniccia, R., Prisco, D., Gensini, G. F., & Serneri, G. G. N. (1996). n-3 PUFA supplementation, monocyte PCA expression and interleukin-6 production. Prostaglandins, Leukotrienes and Essential Fatty Acids, 54(6), 439-444. https://doi.org/10.1016/S0952-3278(96)90028-9