Abstract
Reactive oxygen species formation and release of proinflammatory/pro-atherogenic cytokines, that is, interleukin 1-β and tumor necrosis factor-α, need the activation of the arachidonic acid cascade via the enzyme 5-lipoxygenase (5-Lox). 5-Lox activity and expression are significantly increased in peripheral blood mononuclear cells (PBMCs) of end-stage renal disease (ESRD) patients on maintenance hemodialysis (HD). Diets enriched with n-3 polyunsaturated fatty acids (PUFAs) (ω-3) have been associated to a lower incidence of coronary heart disease (CHD) and a reduction in atherosclerotic lesions. Ω-3 may interfere with the arachidonic acid cascade by inhibiting 5-Lox. Lipid peroxidation, leukotriene B4 (LTB4) production, 5-Lox activity and expression were investigated in PBMC isolated from ESRD patients under maintenance HD before and after a 3-month oral supplementation with ω-3 at a daily dose of 2700 mg of n-3 PUFAs at the average eicosapentaenoic acid/docosaesaenoic acid ratio of 1.2 and finally after a further 3-month washout with no ω-3 supplementation. PBMCs from non-uremic volunteers were also investigated for comparison to normal parameters. Administration of ω-3 reduced significantly lipid peroxidation (P4 synthesis (P
| Original language | English |
|---|---|
| Pages (from-to) | 1450-1454 |
| Number of pages | 5 |
| Journal | Kidney International |
| Volume | 69 |
| Issue number | 8 |
| DOIs | |
| Publication status | Published - Apr 2006 |
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Keywords
- 5-lipoxygenase
- Apoptosis
- Atherosclerosis
- Hemodialysis
- N-3 PUFAS
- Oxidative stress
ASJC Scopus subject areas
- Nephrology
Cite this
N-3 PUFAs reduce oxidative stress in ESRD patients on maintenance HD by inhibiting 5-lipoxygenase activity. / Taccone-Gallucci, M.; Manca-di-Villahermosa, S.; Battistini, L.; Stuffler, R. G.; Tedesco, M.; Maccarrone, M.
In: Kidney International, Vol. 69, No. 8, 04.2006, p. 1450-1454.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - N-3 PUFAs reduce oxidative stress in ESRD patients on maintenance HD by inhibiting 5-lipoxygenase activity
AU - Taccone-Gallucci, M.
AU - Manca-di-Villahermosa, S.
AU - Battistini, L.
AU - Stuffler, R. G.
AU - Tedesco, M.
AU - Maccarrone, M.
PY - 2006/4
Y1 - 2006/4
N2 - Reactive oxygen species formation and release of proinflammatory/pro-atherogenic cytokines, that is, interleukin 1-β and tumor necrosis factor-α, need the activation of the arachidonic acid cascade via the enzyme 5-lipoxygenase (5-Lox). 5-Lox activity and expression are significantly increased in peripheral blood mononuclear cells (PBMCs) of end-stage renal disease (ESRD) patients on maintenance hemodialysis (HD). Diets enriched with n-3 polyunsaturated fatty acids (PUFAs) (ω-3) have been associated to a lower incidence of coronary heart disease (CHD) and a reduction in atherosclerotic lesions. Ω-3 may interfere with the arachidonic acid cascade by inhibiting 5-Lox. Lipid peroxidation, leukotriene B4 (LTB4) production, 5-Lox activity and expression were investigated in PBMC isolated from ESRD patients under maintenance HD before and after a 3-month oral supplementation with ω-3 at a daily dose of 2700 mg of n-3 PUFAs at the average eicosapentaenoic acid/docosaesaenoic acid ratio of 1.2 and finally after a further 3-month washout with no ω-3 supplementation. PBMCs from non-uremic volunteers were also investigated for comparison to normal parameters. Administration of ω-3 reduced significantly lipid peroxidation (P4 synthesis (P
AB - Reactive oxygen species formation and release of proinflammatory/pro-atherogenic cytokines, that is, interleukin 1-β and tumor necrosis factor-α, need the activation of the arachidonic acid cascade via the enzyme 5-lipoxygenase (5-Lox). 5-Lox activity and expression are significantly increased in peripheral blood mononuclear cells (PBMCs) of end-stage renal disease (ESRD) patients on maintenance hemodialysis (HD). Diets enriched with n-3 polyunsaturated fatty acids (PUFAs) (ω-3) have been associated to a lower incidence of coronary heart disease (CHD) and a reduction in atherosclerotic lesions. Ω-3 may interfere with the arachidonic acid cascade by inhibiting 5-Lox. Lipid peroxidation, leukotriene B4 (LTB4) production, 5-Lox activity and expression were investigated in PBMC isolated from ESRD patients under maintenance HD before and after a 3-month oral supplementation with ω-3 at a daily dose of 2700 mg of n-3 PUFAs at the average eicosapentaenoic acid/docosaesaenoic acid ratio of 1.2 and finally after a further 3-month washout with no ω-3 supplementation. PBMCs from non-uremic volunteers were also investigated for comparison to normal parameters. Administration of ω-3 reduced significantly lipid peroxidation (P4 synthesis (P
KW - 5-lipoxygenase
KW - Apoptosis
KW - Atherosclerosis
KW - Hemodialysis
KW - N-3 PUFAS
KW - Oxidative stress
UR - http://www.scopus.com/inward/record.url?scp=33646163680&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33646163680&partnerID=8YFLogxK
U2 - 10.1038/sj.ki.5000291
DO - 10.1038/sj.ki.5000291
M3 - Article
C2 - 16531984
AN - SCOPUS:33646163680
VL - 69
SP - 1450
EP - 1454
JO - Kidney International
JF - Kidney International
SN - 0085-2538
IS - 8
ER -