N-ethyl lidocaine (QX-314) protects striatal neurons against ischemia: An in vitro electrophysiological study

Marta Armogida, Michela Giustizieri, Cristina Zona, Silvia Piccirilli, Robert Nisticò, Nicola B. Mercuri

Research output: Contribution to journalArticlepeer-review

Abstract

In this study, we have investigated the neuroprotective actions of the membrane impermeable, lidocaine analog, N-ethyl lidocaine (QX-314) in the striatum. The effects of this drug were compared with those caused by the strictly-related-compound and sodium channel blocker lidocaine. To address this issue, electrophysiological recordings were performed in striatal slices, in control condition (normoxia) and during combined oxygen and glucose deprivation (in vitro ischemia). Either QX-314 or lidocaine induced, to some extent, a protection of the permanent electrophysiological alteration (field potential loss) caused by a period (12 min) of ischemia. Thus, both compounds permitted a partial recovery of the ischemic depression of the corticostriatal transmission and reduced the amplitude of the ischemic depolarization in medium spiny neurons. However, while QX-314, at the effective concentration of 100 μM, slightly reduced the amplitude of the excitatory field potential and did not affect the current-evoked spikes discharge of medium spiny striatal neurons, equimolar lidocaine depressed the field potential and eliminated repetitive spikes on a depolarizing step. On the basis of these observations, our results suggest the use of QX-314 as a neuroprotective agent in ischemic brain disorders.

Original languageEnglish
Pages (from-to)161-168
Number of pages8
JournalSynapse
Volume64
Issue number2
DOIs
Publication statusPublished - Feb 2010

Keywords

  • Field potentials
  • Ischemia
  • Ischemic depolarization
  • Medium spiny neurons
  • Neuroprotection
  • Patch-clamp
  • Sodium channel blockers
  • Synaptic transmission

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience

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