N-glycan profiles as tools in diagnosis of hepatocellular carcinoma and prediction of healthy human ageing

Valerie Vanhooren, Xue En Liu, Claudio Franceschi, Chun Fang Gao, Claude Libert, Roland Contreras, Cuiying Chen

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Protein glycosylation, the most common form of co-translational modification of proteins, is the enzymatic addition of sugars or oligosaccharides (glycans) to proteins. Protein glycosylation increases the diversity of the functions of proteins encoded in the genome. The result is that different glycomes of the same protein may have different functional, kinetic or physical properties. The glycosylation pathway is largely regulated by the condition of the cells, which means that the sugar chains can be altered by the physiological or pathophysiological condition of the cell. Thus, the type of glycans produced by cells, tissues, or organism could reflect their current physiological state. We determined the N-glycan profiles of serum proteins by using DNA sequencer-based carbohydrate analytical profiling technology. We show that two N-glycan structures (NGA2F and NA2F) present in human blood glycoproteins change with ageing, and that one triantennary glycan (NA3Fb) is correlated with tumor stage in HCC patients. Therefore, examining alterations in serum glycan fingerprint by using our platform could be a suitable tool for monitoring the healthiness of ageing and for the follow-up of pathophysiological conditions such as liver cancer.

Original languageEnglish
Pages (from-to)92-97
Number of pages6
JournalMechanisms of Ageing and Development
Volume130
Issue number1-2
DOIs
Publication statusPublished - Jan 2009

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Polysaccharides
Hepatocellular Carcinoma
Glycosylation
Translational Protein Modification
Proteins
Dermatoglyphics
Liver Neoplasms
Oligosaccharides
Blood Proteins
Glycoproteins
Carbohydrates
Genome
Technology
DNA
Serum
Neoplasms

Keywords

  • Ageing
  • Glycomics
  • Glycosylation
  • Liver cancer
  • N-glycan

ASJC Scopus subject areas

  • Ageing
  • Developmental Biology

Cite this

N-glycan profiles as tools in diagnosis of hepatocellular carcinoma and prediction of healthy human ageing. / Vanhooren, Valerie; Liu, Xue En; Franceschi, Claudio; Gao, Chun Fang; Libert, Claude; Contreras, Roland; Chen, Cuiying.

In: Mechanisms of Ageing and Development, Vol. 130, No. 1-2, 01.2009, p. 92-97.

Research output: Contribution to journalArticle

Vanhooren, Valerie ; Liu, Xue En ; Franceschi, Claudio ; Gao, Chun Fang ; Libert, Claude ; Contreras, Roland ; Chen, Cuiying. / N-glycan profiles as tools in diagnosis of hepatocellular carcinoma and prediction of healthy human ageing. In: Mechanisms of Ageing and Development. 2009 ; Vol. 130, No. 1-2. pp. 92-97.
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