N-Glycomic changes in serum proteins in type 2 diabetes mellitus correlate with complications and with metabolic syndrome parameters

Roberto Testa, Valerie Vanhooren, Anna Rita Bonfigli, Massimo Boemi, Fabiola Olivieri, Antonio Ceriello, Stefano Genovese, Liana Spazzafumo, Vincenzo Borelli, Maria Giulia Bacalini, Stefano Salvioli, Paolo Garagnani, Sylviane Dewaele, Claude Libert, Claudio Franceschi

Research output: Contribution to journalArticlepeer-review

Abstract

Glycosylation, i.e the enzymatic addition of oligosaccharides (or glycans) to proteins and lipids, known as glycosylation, is one of the most common co-/posttranslational modifications of proteins. Many important biological roles of glycoproteins are modulated by N-linked oligosaccharides. As glucose levels can affect the pathways leading to glycosylation of proteins, we investigated whether metabolic syndrome (MS) and type 2 diabetes mellitus (T2DM), pathological conditions characterized by altered glucose levels, are associated with specific modifications in serum N-glycome. Methods We enrolled in the study 562 patients with Type 2 Diabetes Mellitus (T2DM) (mean age 65.6 ±8.2 years) and 599 healthy control subjects (CTRs) (mean age, 58.5±12.4 years). N-glycome was evaluated in serum glycoproteins. Results We found significant changes in N-glycan composition in the sera of T2DM patients. In particular, α(1,6)-linked arm monogalactosylated, core-fucosylated diantennary N-glycans (NG1(6)A2F) were significantly reduced in T2DM compared with CTR subjects. Importantly, they were equally reduced in diabetic patients with and without complications (P

Original languageEnglish
Article numbere0119983
JournalPLoS One
Volume10
Issue number3
DOIs
Publication statusPublished - Mar 20 2015

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

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