N-glycosylation of HIV-gp120 may constrain recognition by T lymphocytes

Patrizia Botarelli, Bronwyn A. Houlden, Nancy L. Haigwood, Catherine Servis, Daniela Montagna, Sergio Abrignani

Research output: Contribution to journalArticle

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Abstract

The HIV envelope protein gp120 is heavily glycosylated, having 55% of its molecular mass contributed by N-linked carbohydrates. We investigated the role of N-glycosylation in presentation of HIV-gp120 to T cells. T cell clones obtained from humans immunized with a recombinant nonglycosylated form of HIV-gp120 (env 2-3) were studied for their ability to recognize both env 2-3 and glycosylated gp120. We found that 20% of CD4+ T cell clones specific for env 2-3 fail to respond to glycosylated gp120 of the same HIV isolate. Using synthetic peptides, we mapped one of the epitopes recognized by such clones to the sequence 292-300 (NESVAINCT), which contains two asparagines that are glycosylated in the native gp120. These findings suggest that N-linked carbohydrates within an epitope can function as hindering structures that limit Ag recognition by T lymphocytes.

Original languageEnglish
Pages (from-to)3128-3132
Number of pages5
JournalJournal of Immunology
Volume147
Issue number9
Publication statusPublished - Nov 1 1991

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HIV Envelope Protein gp120
Glycosylation
T-Lymphocytes
Clone Cells
Epitopes
Carbohydrates
Asparagine
Peptides

ASJC Scopus subject areas

  • Immunology

Cite this

Botarelli, P., Houlden, B. A., Haigwood, N. L., Servis, C., Montagna, D., & Abrignani, S. (1991). N-glycosylation of HIV-gp120 may constrain recognition by T lymphocytes. Journal of Immunology, 147(9), 3128-3132.

N-glycosylation of HIV-gp120 may constrain recognition by T lymphocytes. / Botarelli, Patrizia; Houlden, Bronwyn A.; Haigwood, Nancy L.; Servis, Catherine; Montagna, Daniela; Abrignani, Sergio.

In: Journal of Immunology, Vol. 147, No. 9, 01.11.1991, p. 3128-3132.

Research output: Contribution to journalArticle

Botarelli, P, Houlden, BA, Haigwood, NL, Servis, C, Montagna, D & Abrignani, S 1991, 'N-glycosylation of HIV-gp120 may constrain recognition by T lymphocytes', Journal of Immunology, vol. 147, no. 9, pp. 3128-3132.
Botarelli P, Houlden BA, Haigwood NL, Servis C, Montagna D, Abrignani S. N-glycosylation of HIV-gp120 may constrain recognition by T lymphocytes. Journal of Immunology. 1991 Nov 1;147(9):3128-3132.
Botarelli, Patrizia ; Houlden, Bronwyn A. ; Haigwood, Nancy L. ; Servis, Catherine ; Montagna, Daniela ; Abrignani, Sergio. / N-glycosylation of HIV-gp120 may constrain recognition by T lymphocytes. In: Journal of Immunology. 1991 ; Vol. 147, No. 9. pp. 3128-3132.
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