N-methylformamide induces changes on adhesive properties and lung-colonizing potential of M14 melanoma cells

D. Del Bufalo, C. Leonetti, B. Bucci, C. Amedeo, R. Falcioni, A. Biroccio, G. Zupi

Research output: Contribution to journalArticle

Abstract

We have studied whether N-methylformamide can affect the expression pattern of adhesion molecules and the attachment behaviour of M14 human melanoma cells. The role of N-methylformamide on experimental and spontaneous pulmonary metastases from M14 cells in nude mice was also investigated. We demonstrate that N-methylformamide in vitro pretreatment of M14 cells, although inducing a significant increase in the expression of α2β1, α6β1 and αvβ3 integrin receptors, slightly modifies α5β1 heterodimer and β1 subunit expression. After this modulation, enhancement of cell adhesion to laminin, collagen I, vitronectin and fibrinogen, which is blocked by specific anti-integrin antibodies, also occurs. No changes in binding to fibronectin are observed. In vitro N-methylformamide pretreatment also results in an increased number of experimental nodules and in a decrease in spontaneous metastases. Moreover, in vivo treatment with N-methylformamide significantly reduces the number of spontaneous metastases. Collectively, these data show that N-methylformamide modulates the expression of some adhesion receptors, cell adhesion to laminin, collagen I, vitronectin and fibrinogen as well as the metastatic behaviour of M14 cells. Our data also suggest that the effect of N-methylformamide might be evaluated in combination with antineoplastic agents for the treatment of human melanoma.

Original languageEnglish
Pages (from-to)210-215
Number of pages6
JournalBritish Journal of Cancer
Volume77
Issue number2
Publication statusPublished - 1998

Keywords

  • Human melanoma
  • Integrin
  • Metastasis
  • N-methylformamide

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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