TY - JOUR
T1 - N-myc amplification and cell proliferation rate in human neuroblastoma
AU - Pession, Annalisa
AU - Trerè, Davide
AU - Perri, Patrizia
AU - Rondelli, Roberto
AU - Montanaro, Lorenzo
AU - Mantovani, Walther
AU - Derenzini, Massimo
AU - Paolucci, Guido
PY - 1997/11
Y1 - 1997/11
N2 - In neuroblastoma, N-myc amplification has been found to be strikingly associated with rapid tumour progression and poor prognosis. Recent studies have demonstrated that cell proliferative activity also significantly predicts the clinical outcome in patients with neuroblastoma. In order to define the correlation between N-myc amplification and cell proliferation rate, in the present investigation the two parameters were first assessed in 48 neuroblastoma tumours. N-myc amplification was evaluated in frozen specimens by Southern-blot analysis using the NB 19-21 probe and it was detected in nine patients. Cell proliferative activity was determined by measuring the AgNOR protein area in histological sections selectively stained by silver. The mean AgNOR protein area value of neuroblastomas with N-myc amplification (3.63 ± 1.62 μm2) was not significantly different from that of neuroblastomas without N-myc amplification (2.46 ± 1.57 μm2; P=0.30). On the other hand, both N-myc amplification and AgNOR protein expression were found to be significantly related to the clinical outcome of the disease (P
AB - In neuroblastoma, N-myc amplification has been found to be strikingly associated with rapid tumour progression and poor prognosis. Recent studies have demonstrated that cell proliferative activity also significantly predicts the clinical outcome in patients with neuroblastoma. In order to define the correlation between N-myc amplification and cell proliferation rate, in the present investigation the two parameters were first assessed in 48 neuroblastoma tumours. N-myc amplification was evaluated in frozen specimens by Southern-blot analysis using the NB 19-21 probe and it was detected in nine patients. Cell proliferative activity was determined by measuring the AgNOR protein area in histological sections selectively stained by silver. The mean AgNOR protein area value of neuroblastomas with N-myc amplification (3.63 ± 1.62 μm2) was not significantly different from that of neuroblastomas without N-myc amplification (2.46 ± 1.57 μm2; P=0.30). On the other hand, both N-myc amplification and AgNOR protein expression were found to be significantly related to the clinical outcome of the disease (P
KW - AgNOR protein expression
KW - N-myc amplification
KW - Neuroblastoma cell lines
KW - Neuroblastoma tumours
KW - Prognosis
KW - Proliferation
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U2 - 10.1002/(SICI)1096-9896(199711)183:3<339::AID-PATH935>3.0.CO;2-T
DO - 10.1002/(SICI)1096-9896(199711)183:3<339::AID-PATH935>3.0.CO;2-T
M3 - Article
C2 - 9422991
AN - SCOPUS:0030665836
VL - 183
SP - 339
EP - 344
JO - Journal of Pathology
JF - Journal of Pathology
SN - 0022-3417
IS - 3
ER -