N-myc amplification and cell proliferation rate in human neuroblastoma

Annalisa Pession, Davide Trerè, Patrizia Perri, Roberto Rondelli, Lorenzo Montanaro, Walther Mantovani, Massimo Derenzini, Guido Paolucci

Research output: Contribution to journalArticlepeer-review


In neuroblastoma, N-myc amplification has been found to be strikingly associated with rapid tumour progression and poor prognosis. Recent studies have demonstrated that cell proliferative activity also significantly predicts the clinical outcome in patients with neuroblastoma. In order to define the correlation between N-myc amplification and cell proliferation rate, in the present investigation the two parameters were first assessed in 48 neuroblastoma tumours. N-myc amplification was evaluated in frozen specimens by Southern-blot analysis using the NB 19-21 probe and it was detected in nine patients. Cell proliferative activity was determined by measuring the AgNOR protein area in histological sections selectively stained by silver. The mean AgNOR protein area value of neuroblastomas with N-myc amplification (3.63 ± 1.62 μm2) was not significantly different from that of neuroblastomas without N-myc amplification (2.46 ± 1.57 μm2; P=0.30). On the other hand, both N-myc amplification and AgNOR protein expression were found to be significantly related to the clinical outcome of the disease (P

Original languageEnglish
Pages (from-to)339-344
Number of pages6
JournalJournal of Pathology
Issue number3
Publication statusPublished - Nov 1997


  • AgNOR protein expression
  • N-myc amplification
  • Neuroblastoma cell lines
  • Neuroblastoma tumours
  • Prognosis
  • Proliferation

ASJC Scopus subject areas

  • Pathology and Forensic Medicine


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