N-myc Oncogene amplification in a pediatric case of glioblastoma multiforme

Anna Maria Stenger, Maria Luisa Garrè, Armando Cama, Luciano Andreussi, Massimo Brisigotti, Gian Paolo Tonini, Paolo Cornaglia-Ferraris

Research output: Contribution to journalArticle

Abstract

A sample of a primary brain tumor of glial cell origin was surgically removed from a 7-year-old girl. The histopathological analysis showed a heterogeneous tumor containing highly cellular areas composed of small, poorly differentiated cells with frequent mitoses suggestive of a glioblastoma multiforme. There were also areas presenting as features of lower-grade astrocytoma. Strong immunohistochemical staining for glial fibrillar acidic protein was demonstrated, while vimentin, neurofilament, and S-100 protein were all positive in just the astrocytic part of the tumor. The DNA extracted from a fresh tumor sample at diagnosis was processed by Southern blot analysis and hybridized with a 2.0 kb N-myc oncogene probe recognizing the first intron and the second exon of the human gene. A 20-fold amplification of the oncogene was found. The possible role of such a molecular alteration is discussed in light of the clinical presentation and histopathological features.

Original languageEnglish
Pages (from-to)410-413
Number of pages4
JournalChild's Nervous System
Volume7
Issue number7
DOIs
Publication statusPublished - Nov 1991

Keywords

  • Brain tumors
  • Glioblastoma multiforme
  • N-myc Oncogene
  • Southern blot analysis

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health
  • Clinical Neurology

Fingerprint Dive into the research topics of 'N-myc Oncogene amplification in a pediatric case of glioblastoma multiforme'. Together they form a unique fingerprint.

  • Cite this

    Stenger, A. M., Garrè, M. L., Cama, A., Andreussi, L., Brisigotti, M., Tonini, G. P., & Cornaglia-Ferraris, P. (1991). N-myc Oncogene amplification in a pediatric case of glioblastoma multiforme. Child's Nervous System, 7(7), 410-413. https://doi.org/10.1007/BF00304209