N-Nitrosobutyl(4-hydroxybutyl)amine alpha-hydroxylation by rat liver and urothelial cell homogenates.

L. Airoldi, C. Magagnotti, M. Bonfanti, M. Moret, R. Fanelli

Research output: Contribution to journalArticle

Abstract

Determination of molecular nitrogen formed as a consequence of nitrosamine alpha-hydroxylation provides a useful means for studying the extent of activation of these compounds in target and nontarget organs and tissues. alpha-Hydroxylation in rat liver and urothelial cells was compared using as substrate doubly 15N-labelled N-nitrosobutyl(4-hydroxybutyl)amine (15N-NBHBA), a potent bladder carcinogen in rodents. Both enzyme sources metabolized 15N-NBHBA through the alpha-hydroxylation pathway. 15N2 production was dependent on the amount of substrate incubated. Vmax values for 15N2 production by urothelial cells and by liver postmitochondrial supernatant were 4.47 and 3.21 nmol/mg protein per h, respectively.

Original languageEnglish
Pages (from-to)343-345
Number of pages3
JournalIARC scientific publications
Issue number105
Publication statusPublished - 1991

ASJC Scopus subject areas

  • Medicine(all)

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    Airoldi, L., Magagnotti, C., Bonfanti, M., Moret, M., & Fanelli, R. (1991). N-Nitrosobutyl(4-hydroxybutyl)amine alpha-hydroxylation by rat liver and urothelial cell homogenates. IARC scientific publications, (105), 343-345.