TY - JOUR
T1 - N-oleoylethanolamine inhibits glucosylation of natural ceramides in CHP-100 neuroepithelioma cells
T2 - Possible implications for apoptosis
AU - Spinedi, Angelo
AU - Di Bartolomeo, Sabrina
AU - Piacentini, Mauro
PY - 1999/2/16
Y1 - 1999/2/16
N2 - We report that N-oleoylethanolamine (NOE), widely employed as a ceramidase inhibitor, also inhibits glucosylation of naturally occurring ceramides. When CHP-100 neuroepithelioma cells were exposed for 18 h to non-toxic NOE concentrations (i.e. up to 70 μM), basal incorporation of labelled hexose into glucosylceramide (GlcCer) and higher order neutral glycosphingolipids was significantly inhibited. In cells treated with 30 μM N-hexanoylsphingosine C6-Cer), NOE affected only marginally short-chain glucocerebroside accumulation, but markedly decreased accumulation of glucocerebrosides originating from glucosylation of a long-chain ceramide (Lc-Cer) pool produced upon C6-Cer treatment. Evidence is provided that NOE effects neither are mediated by their effects on ceramidase nor are due to enhanced long-chain GlcCer (Lc-GlcCer) conversion to higher order glycosylated derivatives. NOE inhibition of Lc-GlcCer generation was accompanied by enhanced accumulation of Lc-Cer and by potentiation of apoptosis induced by C6-Cer; the possible causal relationships between these two phenomena are discussed.
AB - We report that N-oleoylethanolamine (NOE), widely employed as a ceramidase inhibitor, also inhibits glucosylation of naturally occurring ceramides. When CHP-100 neuroepithelioma cells were exposed for 18 h to non-toxic NOE concentrations (i.e. up to 70 μM), basal incorporation of labelled hexose into glucosylceramide (GlcCer) and higher order neutral glycosphingolipids was significantly inhibited. In cells treated with 30 μM N-hexanoylsphingosine C6-Cer), NOE affected only marginally short-chain glucocerebroside accumulation, but markedly decreased accumulation of glucocerebrosides originating from glucosylation of a long-chain ceramide (Lc-Cer) pool produced upon C6-Cer treatment. Evidence is provided that NOE effects neither are mediated by their effects on ceramidase nor are due to enhanced long-chain GlcCer (Lc-GlcCer) conversion to higher order glycosylated derivatives. NOE inhibition of Lc-GlcCer generation was accompanied by enhanced accumulation of Lc-Cer and by potentiation of apoptosis induced by C6-Cer; the possible causal relationships between these two phenomena are discussed.
KW - Apoptosis
KW - Ceramide
KW - Glucosylceramide
KW - N-oleoylethanolamine
UR - http://www.scopus.com/inward/record.url?scp=0033573987&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0033573987&partnerID=8YFLogxK
U2 - 10.1006/bbrc.1999.0230
DO - 10.1006/bbrc.1999.0230
M3 - Article
C2 - 10049730
AN - SCOPUS:0033573987
VL - 255
SP - 456
EP - 459
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 2
ER -