N6-isopentenyladenosine Inhibits cell proliferation and Induces apoptosis In a human colon cancer cell line DLD1

Chiara Laezza, Maria Gabriella Caruso, Teresa Gentile, Maria Notarmicola, Anna Maria Malfitano, Tiziana Di Matola, Caterina Messa, Patrizia Gazzerro, Maurizio Bifulco

Research output: Contribution to journalArticle

Abstract

N6-isopentenyladenosine (I6A) is a modified nucleoside with a pentaatomic isopentcnyl derived from mevalonate that induces inhibition of tumor cell proliferation and apoptosis in several tumor cell lines. In this study, we reported that N6-isopentenyladenosine inhibited the proliferation and promotes apoptosis in DLD1 human colon cancer cells. It suppressed the proliferation of cells through inhibition of DNA synthesis, causing a cell cycle arrest that correlated with a decrease in the levels of cyclin E, cyclin A and cyclin D1 and with a concomitant increase in the levels of cyclin-dependent kinase inhibitor p21waf and p27kipl. Moreover, it induced apoptosis through an increase in the number of annexin V-positive cells, a downregulation of antiapoptotic products and caspase-3 activation. The apoptotic effects of N 6-isopentenyladenosine were accompanied by sustained phosphorylation and activation of c-jun N-terminal kinase (JNK) that induced phosphorylation of c-jun. Overall, our data show that JNK, could play an important role in i6A-mediated apoptosis in DLD1 human colon cancer cells

Original languageEnglish
Pages (from-to)1322-1329
Number of pages8
JournalInternational Journal of Cancer
Volume124
Issue number6
DOIs
Publication statusPublished - Mar 15 2009

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Keywords

  • Apoptosis
  • Colon cancer
  • JNK
  • N-isopentenyladenosine

ASJC Scopus subject areas

  • Cancer Research
  • Oncology
  • Medicine(all)

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