NA-Seq: A Discovery Tool for the Analysis of Chromatin Structure and Dynamics during Differentiation

Gaetano Gargiulo, Samuel Levy, Gabriele Bucci, Mauro Romanenghi, Lorenzo Fornasari, Karen Y. Beeson, Susanne M. Goldberg, Matteo Cesaroni, Marco Ballarini, Fabio Santoro, Natalie Bezman, Gianmaria Frigè, Philip D. Gregory, Michael C. Holmes, Robert L. Strausberg, Pier Giuseppe Pelicci, Fyodor D. Urnov, Saverio Minucci

Research output: Contribution to journalArticle

Abstract

It is well established that epigenetic modulation of genome accessibility in chromatin occurs during biological processes. Here we describe a method based on restriction enzymes and next-generation sequencing for identifying accessible DNA elements using a small amount of starting material, and use it to examine myeloid differentiation of primary human CD34+ cells. The accessibility of several classes of cis-regulatory elements was a predictive marker of in vivo DNA binding by transcription factors, and was associated with distinct patterns of histone posttranslational modifications. We also mapped large chromosomal domains with differential accessibility in progenitors and maturing cells. Accessibility became restricted during differentiation, correlating with a decreased number of expressed genes and loss of regulatory potential. Our data suggest that a permissive chromatin structure in multipotent cells is progressively and selectively closed during differentiation, and illustrate the use of our method for the identification of functional cis-regulatory elements.

Original languageEnglish
Pages (from-to)466-481
Number of pages16
JournalDevelopmental Cell
Volume16
Issue number3
DOIs
Publication statusPublished - Mar 17 2009

Keywords

  • DEVBIO
  • DNA

ASJC Scopus subject areas

  • Developmental Biology

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    Gargiulo, G., Levy, S., Bucci, G., Romanenghi, M., Fornasari, L., Beeson, K. Y., Goldberg, S. M., Cesaroni, M., Ballarini, M., Santoro, F., Bezman, N., Frigè, G., Gregory, P. D., Holmes, M. C., Strausberg, R. L., Pelicci, P. G., Urnov, F. D., & Minucci, S. (2009). NA-Seq: A Discovery Tool for the Analysis of Chromatin Structure and Dynamics during Differentiation. Developmental Cell, 16(3), 466-481. https://doi.org/10.1016/j.devcel.2009.02.002