NADPH diaphorase activity is inhibited by EDTA in neurons but not in choroid plexus epithelium

Giuseppe Sancesario, Maria Morello, Roberto Massa, Francesca Fusco, Giorgio Bernardi

Research output: Contribution to journalArticlepeer-review

Abstract

NADPH diaphorase histochemical staining was investigated in rat brain and choroid plexuses. All epithelial cells of the latter as well as some sparse neurons in striatum and cerebral cortex showed strong NADPH reaction product. While staining was homogeneous in neuronal cytoplasm, it was particulate in epithelial cells. Preincubation with EDTA (0.1 mM, 2 h) prevented appearance of NADPH diaphorase reaction in neurons but not in choroid plexuses. These data show that in rat brain two forms of NADPH diaphorase are present; they are specifically localized in neurons and choroidal cells, respectively.

Original languageEnglish
Pages (from-to)101-104
Number of pages4
JournalNeuroscience Letters
Volume158
Issue number1
DOIs
Publication statusPublished - Aug 6 1993

Keywords

  • Choroid plexus
  • Histochemistry
  • NADPH diaphorase
  • NADPH diaphorase neuron
  • Nitric oxide synthase
  • Rat

ASJC Scopus subject areas

  • Neuroscience(all)

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