NADPH oxidase-4 and MATER expressions in granulosa cells: Relationships with ovarian aging

Tullia Maraldi, Elisa Resca, Alessia Nicoli, Francesca Beretti, Manuela Zavatti, Francesco Capodanno, Daria Morini, Stefano Palomba, Giovanni Battista La Sala, Anto De Pol

Research output: Contribution to journalArticle

Abstract

Aims Relevant roles in follicular development and ovulation are played by maternal antigen that embryos require (MATER), product of a maternal effect gene, and by reactive oxygen species (ROS), indispensable for the induction of ovulatory genes. At the moment, the relationship between these two biological systems and their involvement in the ovarian aging have not been still clarified. The aim of the current experimental study was to analyse the age-related changes of the MATER and NOX proteins. Materials and methods MATER and ROS homeostasis was studied in granulosa cells (GCs) and cumulus cells (CCs) of infertile patients who undergone oocyte retrieval for in vitro fertilization cycles using Western blot and confocal immunofluorescence analysis. Samples were obtained from subjects with age ≥ 40 years (cases) and with age ≤ 37 years (controls). Key findings The expression pattern of MATER and NOX observed in GCs was not different from that observed in CCs. High levels of both proteins were detected in the control samples. A significant lower expression of both MATER and NOX4 was observed in the case versus control samples. Significance The expression of MATER and NOX4 proteins are closely related to the follicular development and ovulation with particular regard for ovarian aging.

Original languageEnglish
Pages (from-to)108-114
Number of pages7
JournalLife Sciences
Volume162
DOIs
Publication statusPublished - Oct 1 2016

Fingerprint

Granulosa Cells
NADPH Oxidase
Embryonic Structures
Aging of materials
Mothers
Antigens
Cumulus Cells
Ovulation
Reactive Oxygen Species
Genes
Oocyte Retrieval
Proteins
Biological systems
Fertilization in Vitro
Fluorescent Antibody Technique
Homeostasis
Western Blotting
Cells

Keywords

  • Granulosa cells
  • Infertility
  • MATER
  • NOX4

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Maraldi, T., Resca, E., Nicoli, A., Beretti, F., Zavatti, M., Capodanno, F., ... De Pol, A. (2016). NADPH oxidase-4 and MATER expressions in granulosa cells: Relationships with ovarian aging. Life Sciences, 162, 108-114. https://doi.org/10.1016/j.lfs.2016.08.007

NADPH oxidase-4 and MATER expressions in granulosa cells : Relationships with ovarian aging. / Maraldi, Tullia; Resca, Elisa; Nicoli, Alessia; Beretti, Francesca; Zavatti, Manuela; Capodanno, Francesco; Morini, Daria; Palomba, Stefano; La Sala, Giovanni Battista; De Pol, Anto.

In: Life Sciences, Vol. 162, 01.10.2016, p. 108-114.

Research output: Contribution to journalArticle

Maraldi, T, Resca, E, Nicoli, A, Beretti, F, Zavatti, M, Capodanno, F, Morini, D, Palomba, S, La Sala, GB & De Pol, A 2016, 'NADPH oxidase-4 and MATER expressions in granulosa cells: Relationships with ovarian aging', Life Sciences, vol. 162, pp. 108-114. https://doi.org/10.1016/j.lfs.2016.08.007
Maraldi T, Resca E, Nicoli A, Beretti F, Zavatti M, Capodanno F et al. NADPH oxidase-4 and MATER expressions in granulosa cells: Relationships with ovarian aging. Life Sciences. 2016 Oct 1;162:108-114. https://doi.org/10.1016/j.lfs.2016.08.007
Maraldi, Tullia ; Resca, Elisa ; Nicoli, Alessia ; Beretti, Francesca ; Zavatti, Manuela ; Capodanno, Francesco ; Morini, Daria ; Palomba, Stefano ; La Sala, Giovanni Battista ; De Pol, Anto. / NADPH oxidase-4 and MATER expressions in granulosa cells : Relationships with ovarian aging. In: Life Sciences. 2016 ; Vol. 162. pp. 108-114.
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