Na+/H+ antiporter has different properties in human B lymphocytes according to CD5 expression and malignant phenotype

Dario Ghigo, Gianluca Gaidano, Silvia Treves, Federico Bussolino, Gianpiero Pescarmona, Federico Caligaris-Cappio, Amalia Bosia

Research output: Contribution to journalArticle

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Abstract

In B chronic lymphocytic leukemia (B-CLL) cells, lipopolysaccharide (LPS) and phorbol esters fail to activate the plasma membrane-associated Na+/H+ antiporter and, subsequently, to elicit a rise in cytosolic pH. Since these events are thought to be a prerequisite for LPS-induced proliferation of B normal lymphocytes, we analyzed the kinetic properties of Na+/H+ antiporter in B-CLL cells as compared to both CD5- and CD5+ normal B lymphocytes. In the present work we report that Na+/H+ exchange rate after acid loading is drastically decreased in B-CLL cells, as compared to normal CD5- B lymphocytes, although the antiporter affinity for external Na+ and internal H+ is not significantly different in both cell populations. Kinetic data account for a reduction in the number of operating antiport units in B-CLL. The Na+/H+ antiporter of CD5+ normal B lymphocytes exhibits both an exchange rate and an ion affinity significantly higher than that observed in both CD5+ B-CLL cells and CD5- B normal lymphocytes, thus suggesting a possible explanation for their activated phenotype.

Original languageEnglish
Pages (from-to)583-588
Number of pages6
JournalEuropean Journal of Immunology
Volume21
Issue number3
Publication statusPublished - Mar 1991

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Sodium-Hydrogen Antiporter
B-Cell Chronic Lymphocytic Leukemia
B-Lymphocytes
Phenotype
Lipopolysaccharides
Lymphocytes
Antiporters
Ion Exchange
Ion Transport
Phorbol Esters
Cell Membrane
Acids
Population

ASJC Scopus subject areas

  • Immunology

Cite this

Ghigo, D., Gaidano, G., Treves, S., Bussolino, F., Pescarmona, G., Caligaris-Cappio, F., & Bosia, A. (1991). Na+/H+ antiporter has different properties in human B lymphocytes according to CD5 expression and malignant phenotype. European Journal of Immunology, 21(3), 583-588.

Na+/H+ antiporter has different properties in human B lymphocytes according to CD5 expression and malignant phenotype. / Ghigo, Dario; Gaidano, Gianluca; Treves, Silvia; Bussolino, Federico; Pescarmona, Gianpiero; Caligaris-Cappio, Federico; Bosia, Amalia.

In: European Journal of Immunology, Vol. 21, No. 3, 03.1991, p. 583-588.

Research output: Contribution to journalArticle

Ghigo, D, Gaidano, G, Treves, S, Bussolino, F, Pescarmona, G, Caligaris-Cappio, F & Bosia, A 1991, 'Na+/H+ antiporter has different properties in human B lymphocytes according to CD5 expression and malignant phenotype', European Journal of Immunology, vol. 21, no. 3, pp. 583-588.
Ghigo, Dario ; Gaidano, Gianluca ; Treves, Silvia ; Bussolino, Federico ; Pescarmona, Gianpiero ; Caligaris-Cappio, Federico ; Bosia, Amalia. / Na+/H+ antiporter has different properties in human B lymphocytes according to CD5 expression and malignant phenotype. In: European Journal of Immunology. 1991 ; Vol. 21, No. 3. pp. 583-588.
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