Nano-targeting of mucosal addressin cell adhesion molecule-1 identifies bowel inflammation foci in murine model

Marta Truffi; Miriam Colombo; Jesus Peñaranda-Avila; Luca Sorrentino; Francesco Colombo; Matteo Monieri; Veronica Collico; Pietro Zerbi; Erika Longhi; Raffaele Allevi; Davide Prosperi; Fabio Corsi

doi:10.2217/nnm-2017-0004

Nano-targeting of mucosal addressin cell adhesion molecule-1 identifies bowel inflammation foci in murine model

Marta Truffi, Miriam Colombo, Jesus Peñaranda-Avila, Luca Sorrentino, Francesco Colombo, Matteo Monieri, Veronica Collico, Pietro Zerbi, Erika Longhi, Raffaele Allevi, Davide Prosperi, Fabio Corsi

Istituti Clinici Scientifici Maugeri Spa – Società Benefit

Research output: Contribution to journal › Article › peer-review

Abstract

AIM: We investigate MAdCAM-1 as a reliable target to detect active bowel inflammation for selective noninvasive nanodiagnostics.

MATERIALS & METHODS: We coupled anti-MAdCAM-1 antibodies to manganese oxide nanoparticles, and analyzed nanoconjugate biodistribution and safety in murine model of inflammatory bowel disease by imaging and histology.

RESULTS: Nanoparticles were stable and nontoxic. Upon administration in colitic mice, anti-MAdCAM-1 functionalized nanoparticles preferentially localized in the inflamed bowel, whereas untargeted nanoparticles were more rapidly washed out. Nanoparticles did not induce lesions in nontarget organs.

CONCLUSION: Anti-MAdCAM-1 functionalized nanoparticles detected active bowel inflammation foci, accurately following MAdCAM-1 expression pattern. These nanoconjugates could be a promising noninvasive imaging system for an early and accurate follow-up in patients affected by acute colitis.

Original language	English
Pages (from-to)	1547-1560
Number of pages	14
Journal	Nanomedicine
Volume	12
Issue number	13
DOIs	https://doi.org/10.2217/nnm-2017-0004
Publication status	Published - Jul 2017

Keywords

Animals
Antibodies
Cell Survival
Colitis
Hemolysis
Humans
Immunoglobulins
Male
Manganese Compounds
Metal Nanoparticles
Mice
Mice, Inbred C57BL
Mucoproteins
Oxides
Particle Size
Surface Properties
Tissue Distribution
Journal Article

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10.2217/nnm-2017-0004

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Nano-targeting of mucosal addressin cell adhesion molecule-1 identifies bowel inflammation foci in murine model. / Truffi, Marta; Colombo, Miriam; Peñaranda-Avila, Jesus; Sorrentino, Luca; Colombo, Francesco; Monieri, Matteo; Collico, Veronica; Zerbi, Pietro; Longhi, Erika; Allevi, Raffaele; Prosperi, Davide; Corsi, Fabio.

In: Nanomedicine, Vol. 12, No. 13, 07.2017, p. 1547-1560.

Research output: Contribution to journal › Article › peer-review

Truffi, Marta ; Colombo, Miriam ; Peñaranda-Avila, Jesus ; Sorrentino, Luca ; Colombo, Francesco ; Monieri, Matteo ; Collico, Veronica ; Zerbi, Pietro ; Longhi, Erika ; Allevi, Raffaele ; Prosperi, Davide ; Corsi, Fabio. / Nano-targeting of mucosal addressin cell adhesion molecule-1 identifies bowel inflammation foci in murine model. In: Nanomedicine. 2017 ; Vol. 12, No. 13. pp. 1547-1560.

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abstract = "AIM: We investigate MAdCAM-1 as a reliable target to detect active bowel inflammation for selective noninvasive nanodiagnostics.MATERIALS & METHODS: We coupled anti-MAdCAM-1 antibodies to manganese oxide nanoparticles, and analyzed nanoconjugate biodistribution and safety in murine model of inflammatory bowel disease by imaging and histology.RESULTS: Nanoparticles were stable and nontoxic. Upon administration in colitic mice, anti-MAdCAM-1 functionalized nanoparticles preferentially localized in the inflamed bowel, whereas untargeted nanoparticles were more rapidly washed out. Nanoparticles did not induce lesions in nontarget organs.CONCLUSION: Anti-MAdCAM-1 functionalized nanoparticles detected active bowel inflammation foci, accurately following MAdCAM-1 expression pattern. These nanoconjugates could be a promising noninvasive imaging system for an early and accurate follow-up in patients affected by acute colitis.",

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author = "Marta Truffi and Miriam Colombo and Jesus Pe{\~n}aranda-Avila and Luca Sorrentino and Francesco Colombo and Matteo Monieri and Veronica Collico and Pietro Zerbi and Erika Longhi and Raffaele Allevi and Davide Prosperi and Fabio Corsi",

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AU - Colombo, Miriam

AU - Peñaranda-Avila, Jesus

AU - Sorrentino, Luca

AU - Colombo, Francesco

AU - Monieri, Matteo

AU - Collico, Veronica

AU - Zerbi, Pietro

AU - Longhi, Erika

AU - Allevi, Raffaele

AU - Prosperi, Davide

AU - Corsi, Fabio

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N2 - AIM: We investigate MAdCAM-1 as a reliable target to detect active bowel inflammation for selective noninvasive nanodiagnostics.MATERIALS & METHODS: We coupled anti-MAdCAM-1 antibodies to manganese oxide nanoparticles, and analyzed nanoconjugate biodistribution and safety in murine model of inflammatory bowel disease by imaging and histology.RESULTS: Nanoparticles were stable and nontoxic. Upon administration in colitic mice, anti-MAdCAM-1 functionalized nanoparticles preferentially localized in the inflamed bowel, whereas untargeted nanoparticles were more rapidly washed out. Nanoparticles did not induce lesions in nontarget organs.CONCLUSION: Anti-MAdCAM-1 functionalized nanoparticles detected active bowel inflammation foci, accurately following MAdCAM-1 expression pattern. These nanoconjugates could be a promising noninvasive imaging system for an early and accurate follow-up in patients affected by acute colitis.

AB - AIM: We investigate MAdCAM-1 as a reliable target to detect active bowel inflammation for selective noninvasive nanodiagnostics.MATERIALS & METHODS: We coupled anti-MAdCAM-1 antibodies to manganese oxide nanoparticles, and analyzed nanoconjugate biodistribution and safety in murine model of inflammatory bowel disease by imaging and histology.RESULTS: Nanoparticles were stable and nontoxic. Upon administration in colitic mice, anti-MAdCAM-1 functionalized nanoparticles preferentially localized in the inflamed bowel, whereas untargeted nanoparticles were more rapidly washed out. Nanoparticles did not induce lesions in nontarget organs.CONCLUSION: Anti-MAdCAM-1 functionalized nanoparticles detected active bowel inflammation foci, accurately following MAdCAM-1 expression pattern. These nanoconjugates could be a promising noninvasive imaging system for an early and accurate follow-up in patients affected by acute colitis.

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KW - Mice, Inbred C57BL

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KW - Oxides

KW - Particle Size

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Nano-targeting of mucosal addressin cell adhesion molecule-1 identifies bowel inflammation foci in murine model

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