Nano-targeting of mucosal addressin cell adhesion molecule-1 identifies bowel inflammation foci in murine model

Marta Truffi, Miriam Colombo, Jesus Peñaranda-Avila, Luca Sorrentino, Francesco Colombo, Matteo Monieri, Veronica Collico, Pietro Zerbi, Erika Longhi, Raffaele Allevi, Davide Prosperi, Fabio Corsi

Research output: Contribution to journalArticlepeer-review

Abstract

AIM: We investigate MAdCAM-1 as a reliable target to detect active bowel inflammation for selective noninvasive nanodiagnostics.

MATERIALS & METHODS: We coupled anti-MAdCAM-1 antibodies to manganese oxide nanoparticles, and analyzed nanoconjugate biodistribution and safety in murine model of inflammatory bowel disease by imaging and histology.

RESULTS: Nanoparticles were stable and nontoxic. Upon administration in colitic mice, anti-MAdCAM-1 functionalized nanoparticles preferentially localized in the inflamed bowel, whereas untargeted nanoparticles were more rapidly washed out. Nanoparticles did not induce lesions in nontarget organs.

CONCLUSION: Anti-MAdCAM-1 functionalized nanoparticles detected active bowel inflammation foci, accurately following MAdCAM-1 expression pattern. These nanoconjugates could be a promising noninvasive imaging system for an early and accurate follow-up in patients affected by acute colitis.

Original languageEnglish
Pages (from-to)1547-1560
Number of pages14
JournalNanomedicine
Volume12
Issue number13
DOIs
Publication statusPublished - Jul 2017

Keywords

  • Animals
  • Antibodies
  • Cell Survival
  • Colitis
  • Hemolysis
  • Humans
  • Immunoglobulins
  • Male
  • Manganese Compounds
  • Metal Nanoparticles
  • Mice
  • Mice, Inbred C57BL
  • Mucoproteins
  • Oxides
  • Particle Size
  • Surface Properties
  • Tissue Distribution
  • Journal Article

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