NanoCAGE analysis of the mouse olfactory epithelium identifies the expression of vomeronasal receptors and of proximal LINE elements

Giovanni Pascarella, Dejan Lazarevic, Charles Plessy, Nicolas Bertin, Altuna Akalin, Christina Vlachouli, Roberto Simone, Geoffrey J. Faulkner, Silvia Zucchelli, Jun Kawai, Carsten O. Daub, Yoshihide Hayashizaki, Boris Lenhard, Piero Carninci, Stefano Gustincich

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

By coupling laser capture microdissection to nanoCAGE technology and next-generation sequencing we have identified the genome-wide collection of active promoters in the mouse Main Olfactory Epithelium (MOE). Transcription start sites (TSSs) for the large majority of Olfactory Receptors (ORs) have been previously mapped increasing our understanding of their promoter architecture. Here we show that in our nanoCAGE libraries of the mouse MOE we detect a large number of tags mapped in loci hosting Type-1 and Type-2 Vomeronasal Receptors genes (V1Rs and V2Rs). These loci also show a massive expression of Long Interspersed Nuclear Elements (LINEs). We have validated the expression of selected receptors detected by nanoCAGE with in situ hybridization, RT-PCR and qRT-PCR. This work extends the repertory of receptors capable of sensing chemical signals in the MOE, suggesting intriguing interplays between MOE and VNO for pheromone processing and positioning transcribed LINEs as candidate regulatory RNAs for VRs expression.

Original languageEnglish
Article number41
JournalFrontiers in Cellular Neuroscience
Volume8
Issue numberFEB
DOIs
Publication statusPublished - Feb 18 2014

Fingerprint

Olfactory Mucosa
Odorant Receptors
Laser Capture Microdissection
Polymerase Chain Reaction
Transcription Initiation Site
Pheromones
Libraries
In Situ Hybridization
Genome
RNA
Technology
Genes

Keywords

  • Main olfactory epithelium
  • MOE
  • V1Rs
  • V2Rs
  • VNO
  • Vomeronasal organ
  • Vomeronasal receptors

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience

Cite this

NanoCAGE analysis of the mouse olfactory epithelium identifies the expression of vomeronasal receptors and of proximal LINE elements. / Pascarella, Giovanni; Lazarevic, Dejan; Plessy, Charles; Bertin, Nicolas; Akalin, Altuna; Vlachouli, Christina; Simone, Roberto; Faulkner, Geoffrey J.; Zucchelli, Silvia; Kawai, Jun; Daub, Carsten O.; Hayashizaki, Yoshihide; Lenhard, Boris; Carninci, Piero; Gustincich, Stefano.

In: Frontiers in Cellular Neuroscience, Vol. 8, No. FEB, 41, 18.02.2014.

Research output: Contribution to journalArticle

Pascarella, G, Lazarevic, D, Plessy, C, Bertin, N, Akalin, A, Vlachouli, C, Simone, R, Faulkner, GJ, Zucchelli, S, Kawai, J, Daub, CO, Hayashizaki, Y, Lenhard, B, Carninci, P & Gustincich, S 2014, 'NanoCAGE analysis of the mouse olfactory epithelium identifies the expression of vomeronasal receptors and of proximal LINE elements', Frontiers in Cellular Neuroscience, vol. 8, no. FEB, 41. https://doi.org/10.3389/fncel.2014.00041
Pascarella, Giovanni ; Lazarevic, Dejan ; Plessy, Charles ; Bertin, Nicolas ; Akalin, Altuna ; Vlachouli, Christina ; Simone, Roberto ; Faulkner, Geoffrey J. ; Zucchelli, Silvia ; Kawai, Jun ; Daub, Carsten O. ; Hayashizaki, Yoshihide ; Lenhard, Boris ; Carninci, Piero ; Gustincich, Stefano. / NanoCAGE analysis of the mouse olfactory epithelium identifies the expression of vomeronasal receptors and of proximal LINE elements. In: Frontiers in Cellular Neuroscience. 2014 ; Vol. 8, No. FEB.
@article{11f2c0d05845418c990ae94c2153d51d,
title = "NanoCAGE analysis of the mouse olfactory epithelium identifies the expression of vomeronasal receptors and of proximal LINE elements",
abstract = "By coupling laser capture microdissection to nanoCAGE technology and next-generation sequencing we have identified the genome-wide collection of active promoters in the mouse Main Olfactory Epithelium (MOE). Transcription start sites (TSSs) for the large majority of Olfactory Receptors (ORs) have been previously mapped increasing our understanding of their promoter architecture. Here we show that in our nanoCAGE libraries of the mouse MOE we detect a large number of tags mapped in loci hosting Type-1 and Type-2 Vomeronasal Receptors genes (V1Rs and V2Rs). These loci also show a massive expression of Long Interspersed Nuclear Elements (LINEs). We have validated the expression of selected receptors detected by nanoCAGE with in situ hybridization, RT-PCR and qRT-PCR. This work extends the repertory of receptors capable of sensing chemical signals in the MOE, suggesting intriguing interplays between MOE and VNO for pheromone processing and positioning transcribed LINEs as candidate regulatory RNAs for VRs expression.",
keywords = "Main olfactory epithelium, MOE, V1Rs, V2Rs, VNO, Vomeronasal organ, Vomeronasal receptors",
author = "Giovanni Pascarella and Dejan Lazarevic and Charles Plessy and Nicolas Bertin and Altuna Akalin and Christina Vlachouli and Roberto Simone and Faulkner, {Geoffrey J.} and Silvia Zucchelli and Jun Kawai and Daub, {Carsten O.} and Yoshihide Hayashizaki and Boris Lenhard and Piero Carninci and Stefano Gustincich",
year = "2014",
month = "2",
day = "18",
doi = "10.3389/fncel.2014.00041",
language = "English",
volume = "8",
journal = "Frontiers in Cellular Neuroscience",
issn = "1662-5102",
publisher = "Frontiers Media S.A.",
number = "FEB",

}

TY - JOUR

T1 - NanoCAGE analysis of the mouse olfactory epithelium identifies the expression of vomeronasal receptors and of proximal LINE elements

AU - Pascarella, Giovanni

AU - Lazarevic, Dejan

AU - Plessy, Charles

AU - Bertin, Nicolas

AU - Akalin, Altuna

AU - Vlachouli, Christina

AU - Simone, Roberto

AU - Faulkner, Geoffrey J.

AU - Zucchelli, Silvia

AU - Kawai, Jun

AU - Daub, Carsten O.

AU - Hayashizaki, Yoshihide

AU - Lenhard, Boris

AU - Carninci, Piero

AU - Gustincich, Stefano

PY - 2014/2/18

Y1 - 2014/2/18

N2 - By coupling laser capture microdissection to nanoCAGE technology and next-generation sequencing we have identified the genome-wide collection of active promoters in the mouse Main Olfactory Epithelium (MOE). Transcription start sites (TSSs) for the large majority of Olfactory Receptors (ORs) have been previously mapped increasing our understanding of their promoter architecture. Here we show that in our nanoCAGE libraries of the mouse MOE we detect a large number of tags mapped in loci hosting Type-1 and Type-2 Vomeronasal Receptors genes (V1Rs and V2Rs). These loci also show a massive expression of Long Interspersed Nuclear Elements (LINEs). We have validated the expression of selected receptors detected by nanoCAGE with in situ hybridization, RT-PCR and qRT-PCR. This work extends the repertory of receptors capable of sensing chemical signals in the MOE, suggesting intriguing interplays between MOE and VNO for pheromone processing and positioning transcribed LINEs as candidate regulatory RNAs for VRs expression.

AB - By coupling laser capture microdissection to nanoCAGE technology and next-generation sequencing we have identified the genome-wide collection of active promoters in the mouse Main Olfactory Epithelium (MOE). Transcription start sites (TSSs) for the large majority of Olfactory Receptors (ORs) have been previously mapped increasing our understanding of their promoter architecture. Here we show that in our nanoCAGE libraries of the mouse MOE we detect a large number of tags mapped in loci hosting Type-1 and Type-2 Vomeronasal Receptors genes (V1Rs and V2Rs). These loci also show a massive expression of Long Interspersed Nuclear Elements (LINEs). We have validated the expression of selected receptors detected by nanoCAGE with in situ hybridization, RT-PCR and qRT-PCR. This work extends the repertory of receptors capable of sensing chemical signals in the MOE, suggesting intriguing interplays between MOE and VNO for pheromone processing and positioning transcribed LINEs as candidate regulatory RNAs for VRs expression.

KW - Main olfactory epithelium

KW - MOE

KW - V1Rs

KW - V2Rs

KW - VNO

KW - Vomeronasal organ

KW - Vomeronasal receptors

UR - http://www.scopus.com/inward/record.url?scp=84894048516&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84894048516&partnerID=8YFLogxK

U2 - 10.3389/fncel.2014.00041

DO - 10.3389/fncel.2014.00041

M3 - Article

AN - SCOPUS:84894048516

VL - 8

JO - Frontiers in Cellular Neuroscience

JF - Frontiers in Cellular Neuroscience

SN - 1662-5102

IS - FEB

M1 - 41

ER -