Nanocapsules: Coating for Living Cells

Silke Krol, Alberto Diaspro, Raffaella Magrassi, Paola Ballario, Benedetto Grimaldi, Patrizia Filetici, Prisca Ornaghi, Paola Ramoino, Alessandra Gliozzi

Research output: Contribution to journalArticlepeer-review

Abstract

One of the most promising tools for future applications in science and medicine is the use of nanotechnologies. Especially self-assembly systems, e.g., polyelectrolyte (PE) capsules prepared by means of the layer-by-layer technique with tailored properties, fulfill the requirements for nano-organized systems in a satisfactory manner. The nano-organized shells are suitable as coating for living cells or artificial tissue to prevent immune response. With these shells, material can be delivered to predefined organs. In this paper, some preliminary results are presented, giving a broad overview over the possibilities to use nano-organized capsules. Based on the observations that the cells while duplicating break the capsule a mutant yeast strain (Saccharomyces cerevisiae), which express GFP-tubulin under galactose promotion, was investigated by means of confocal laser scanning microscopy. The measurements reveal an increased surface charge in the region of buds developed prior encapsulation. In order to test the used PE pair for cytotoxicity, germinating conidia of the fungi Neurospora crassa were coated. The investigation with fluorescence microscopy shows a variation in the surface charge for the growing region and the conidium poles. The capsules exhibit interesting properties as valuable tool in science and a promising candidate for application in the field of medicine.

Original languageEnglish
Pages (from-to)32-38
Number of pages7
JournalIEEE Transactions on Nanobioscience
Volume3
Issue number1
DOIs
Publication statusPublished - Mar 2004

Keywords

  • Confocal laser scanning microscopy
  • Living cells
  • Nanocapsule
  • Polyelectrolyte (pe)

ASJC Scopus subject areas

  • Engineering(all)
  • Bioengineering
  • Computer Science Applications
  • Electrical and Electronic Engineering
  • Pharmaceutical Science
  • Medicine (miscellaneous)
  • Biotechnology
  • Biomedical Engineering

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