TY - JOUR
T1 - Nanoscale quantification of intracellular element concentration by X-ray fluorescence microscopy combined with X-ray phase contrast nanotomography
AU - Gramaccioni, Chiara
AU - Yang, Yang
AU - Procopio, Alessandra
AU - Pacureanu, Alexandra
AU - Bohic, Sylvain
AU - Malucelli, Emil
AU - Iotti, Stefano
AU - Farruggia, Giovanna
AU - Bukreeva, Inna
AU - Notargiacomo, Andrea
AU - Fratini, Michela
AU - Valenti, Piera
AU - Rosa, Luigi
AU - Berlutti, Francesca
AU - Cloetens, Peter
AU - Lagomarsino, Stefano
PY - 2017/12/13
Y1 - 2017/12/13
N2 - We present here a correlative X-ray microscopy approach for quantitative single cell imaging of molar concentrations. By combining the elemental content provided by X-ray fluorescence microscopy and the morphology information extracted from X-ray phase nanotomography, we determine the intracellular molarity distributions. This correlative method was demonstrated on a freeze-dried human phagocytic cell to obtain the absolute elemental concentration maps of K, P, and Fe. The cell morphology results showed a very good agreement with atomic-force microscopy measurements. This work opens the way for non-destructive single cell chemical analysis down to the sub-cellular level using exclusively synchrotron radiation techniques. It will be of high interest in the case where it is difficult to access the morphology using atomic-force microscopy, for example, on frozen-hydrated cells or tissues.
AB - We present here a correlative X-ray microscopy approach for quantitative single cell imaging of molar concentrations. By combining the elemental content provided by X-ray fluorescence microscopy and the morphology information extracted from X-ray phase nanotomography, we determine the intracellular molarity distributions. This correlative method was demonstrated on a freeze-dried human phagocytic cell to obtain the absolute elemental concentration maps of K, P, and Fe. The cell morphology results showed a very good agreement with atomic-force microscopy measurements. This work opens the way for non-destructive single cell chemical analysis down to the sub-cellular level using exclusively synchrotron radiation techniques. It will be of high interest in the case where it is difficult to access the morphology using atomic-force microscopy, for example, on frozen-hydrated cells or tissues.
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U2 - 10.1063/1.5008834
DO - 10.1063/1.5008834
M3 - Article
AN - SCOPUS:85041431846
VL - 112
JO - Applied Physics Letters
JF - Applied Physics Letters
SN - 0003-6951
IS - 5
M1 - 053701
ER -