Nanoscale quantification of intracellular element concentration by X-ray fluorescence microscopy combined with X-ray phase contrast nanotomography

Chiara Gramaccioni, Yang Yang, Alessandra Procopio, Alexandra Pacureanu, Sylvain Bohic, Emil Malucelli, Stefano Iotti, Giovanna Farruggia, Inna Bukreeva, Andrea Notargiacomo, Michela Fratini, Piera Valenti, Luigi Rosa, Francesca Berlutti, Peter Cloetens, Stefano Lagomarsino

Research output: Contribution to journalArticlepeer-review

Abstract

We present here a correlative X-ray microscopy approach for quantitative single cell imaging of molar concentrations. By combining the elemental content provided by X-ray fluorescence microscopy and the morphology information extracted from X-ray phase nanotomography, we determine the intracellular molarity distributions. This correlative method was demonstrated on a freeze-dried human phagocytic cell to obtain the absolute elemental concentration maps of K, P, and Fe. The cell morphology results showed a very good agreement with atomic-force microscopy measurements. This work opens the way for non-destructive single cell chemical analysis down to the sub-cellular level using exclusively synchrotron radiation techniques. It will be of high interest in the case where it is difficult to access the morphology using atomic-force microscopy, for example, on frozen-hydrated cells or tissues.

Original languageEnglish
Article number053701
JournalApplied Physics Letters
Volume112
Issue number5
DOIs
Publication statusE-pub ahead of print - Dec 13 2017

ASJC Scopus subject areas

  • Physics and Astronomy (miscellaneous)

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