Nanostructured TiO2 surfaces promote polarized activation of microglia, but not astrocytes, toward a proinflammatory profile

Silvia De Astis, Irene Corradini, Raffaella Morini, Simona Rodighiero, Romana Tomasoni, Cristina Lenardi, Claudia Verderio, Paolo Milani, Michela Matteoli

Research output: Contribution to journalArticle

Abstract

Activation of glial cells, including astrocytes and microglia, has been implicated in the inflammatory responses underlying brain injury and neurodegenerative diseases including Alzheimer's and Parkinson's diseases. The classic activation state (M1) is characterized by high capacity to present antigens, high production of nitric oxide (NO) and reactive oxygen species (ROS) and proinflammatory cytokines. Classically activated cells act as potent effectors that drive the inflammatory response and may mediate detrimental effects on neural cells. The second phenotype (M2) is an alternative, apparently beneficial, activation state, more related to a fine tuning of inflammation, scavenging of debris, promotion of angiogenesis, tissue remodeling and repair. Specific environmental chemical signals are able to induce these different polarization states. We provide here evidence that nanostructured substrates are able, exclusively in virtue of their physical properties, to push microglia toward the proinflammatory activation phenotype, with an efficacy which reflects the graded nanoscale rugosity. The acquisition of a proinflammatory phenotype appears specific for microglia and not astrocytes, indicating that these two cell types, although sharing common innate immune responses, respond differently to external physical stimuli.

Original languageEnglish
Pages (from-to)10963-10974
Number of pages12
JournalNanoscale
Volume5
Issue number22
DOIs
Publication statusPublished - Nov 21 2013

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Chemical activation
Neurodegenerative diseases
Scavenging
Nitric oxide
Antigens
Debris
Reactive Oxygen Species
Brain
Nitric Oxide
Repair
Tuning
Physical properties
Polarization
Tissue
Cytokines
Oxygen
Astrocytes
Microglia
Substrates

ASJC Scopus subject areas

  • Materials Science(all)

Cite this

Nanostructured TiO2 surfaces promote polarized activation of microglia, but not astrocytes, toward a proinflammatory profile. / De Astis, Silvia; Corradini, Irene; Morini, Raffaella; Rodighiero, Simona; Tomasoni, Romana; Lenardi, Cristina; Verderio, Claudia; Milani, Paolo; Matteoli, Michela.

In: Nanoscale, Vol. 5, No. 22, 21.11.2013, p. 10963-10974.

Research output: Contribution to journalArticle

De Astis, Silvia ; Corradini, Irene ; Morini, Raffaella ; Rodighiero, Simona ; Tomasoni, Romana ; Lenardi, Cristina ; Verderio, Claudia ; Milani, Paolo ; Matteoli, Michela. / Nanostructured TiO2 surfaces promote polarized activation of microglia, but not astrocytes, toward a proinflammatory profile. In: Nanoscale. 2013 ; Vol. 5, No. 22. pp. 10963-10974.
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