Nanostructured TiO2 surfaces promote human bone marrow mesenchymal stem cells differentiation to osteoblasts

M. Vercellino, Gabriele Ceccarelli, Francesco Cristofaro, Martina Balli, Federico Bertoglio, G. Bruni, L. Benedetti, Maria Antonietta Avanzini, Marcello Imbriani, Livia Visai

Research output: Contribution to journalArticle

Abstract

Micro-and nano-patterning/modification are emerging strategies to improve surfaces properties that may influence critically cells adherence and differentiation. Aim of this work was to study the in vitro biological reactivity of human bone marrow mesenchymal stem cells (hBMSCs) to a nanostructured titanium dioxide (TiO2) surface in comparison to a coverglass (Glass) in two different culture conditions: with (osteogenic medium (OM)) and without (proliferative medium (PM)) osteogenic factors. To evaluate cell adhesion, hBMSCs phosphorylated focal adhesion kinase (pFAK) foci were analyzed by confocal laser scanning microscopy (CLSM) at 24 h: the TiO2 surface showed a higher number of pFAK foci with respect to Glass. The hBMSCs differentiation to osteoblasts was evaluated in both PM and OM culture conditions by enzyme-linked immunosorbent assay (ELISA), CLSM and real-time quantitative reverse transcription PCR (qRT-PCR) at 28 days. In comparison with Glass, TiO2 surface in combination with OM conditions increased the content of extracellular bone proteins, calcium deposition and alkaline phosphatase activity. The qRT-PCR analysis revealed, both in PM and OM, that TiO2 surface increased at seven and 28 days the expression of osteogenic genes. All together, these results demonstrate the capability of TiO2 nanostructured surface to promote hBMSCs osteoblast differentiation and its potentiality in biomedical applications.

Original languageEnglish
Article number124
JournalNanomaterials
Volume6
Issue number7
DOIs
Publication statusPublished - Jul 1 2016

Fingerprint

Osteoblasts
Stem cells
Bone
Focal Adhesion Protein-Tyrosine Kinases
Transcription
Glass
Microscopic examination
Adhesion
Scanning
Immunosorbents
Lasers
Cell adhesion
Phosphatases
Cell culture
Titanium dioxide
Surface properties
Alkaline Phosphatase
Culture Media
Calcium
Assays

Keywords

  • Biomedical applications
  • Bone tissue engineering and regeneration
  • Human bone marrow mesenchymal stem cells
  • Nano-patterning modification
  • Titanium dioxide surface

ASJC Scopus subject areas

  • Materials Science(all)
  • Chemical Engineering(all)

Cite this

Nanostructured TiO2 surfaces promote human bone marrow mesenchymal stem cells differentiation to osteoblasts. / Vercellino, M.; Ceccarelli, Gabriele; Cristofaro, Francesco; Balli, Martina; Bertoglio, Federico; Bruni, G.; Benedetti, L.; Avanzini, Maria Antonietta; Imbriani, Marcello; Visai, Livia.

In: Nanomaterials, Vol. 6, No. 7, 124, 01.07.2016.

Research output: Contribution to journalArticle

Vercellino M, Ceccarelli G, Cristofaro F, Balli M, Bertoglio F, Bruni G et al. Nanostructured TiO2 surfaces promote human bone marrow mesenchymal stem cells differentiation to osteoblasts. Nanomaterials. 2016 Jul 1;6(7). 124. https://doi.org/10.3390/nano6070124
Vercellino, M. ; Ceccarelli, Gabriele ; Cristofaro, Francesco ; Balli, Martina ; Bertoglio, Federico ; Bruni, G. ; Benedetti, L. ; Avanzini, Maria Antonietta ; Imbriani, Marcello ; Visai, Livia. / Nanostructured TiO2 surfaces promote human bone marrow mesenchymal stem cells differentiation to osteoblasts. In: Nanomaterials. 2016 ; Vol. 6, No. 7.
@article{eb290ca8fab643d99cfbbe849ef5f00f,
title = "Nanostructured TiO2 surfaces promote human bone marrow mesenchymal stem cells differentiation to osteoblasts",
abstract = "Micro-and nano-patterning/modification are emerging strategies to improve surfaces properties that may influence critically cells adherence and differentiation. Aim of this work was to study the in vitro biological reactivity of human bone marrow mesenchymal stem cells (hBMSCs) to a nanostructured titanium dioxide (TiO2) surface in comparison to a coverglass (Glass) in two different culture conditions: with (osteogenic medium (OM)) and without (proliferative medium (PM)) osteogenic factors. To evaluate cell adhesion, hBMSCs phosphorylated focal adhesion kinase (pFAK) foci were analyzed by confocal laser scanning microscopy (CLSM) at 24 h: the TiO2 surface showed a higher number of pFAK foci with respect to Glass. The hBMSCs differentiation to osteoblasts was evaluated in both PM and OM culture conditions by enzyme-linked immunosorbent assay (ELISA), CLSM and real-time quantitative reverse transcription PCR (qRT-PCR) at 28 days. In comparison with Glass, TiO2 surface in combination with OM conditions increased the content of extracellular bone proteins, calcium deposition and alkaline phosphatase activity. The qRT-PCR analysis revealed, both in PM and OM, that TiO2 surface increased at seven and 28 days the expression of osteogenic genes. All together, these results demonstrate the capability of TiO2 nanostructured surface to promote hBMSCs osteoblast differentiation and its potentiality in biomedical applications.",
keywords = "Biomedical applications, Bone tissue engineering and regeneration, Human bone marrow mesenchymal stem cells, Nano-patterning modification, Titanium dioxide surface",
author = "M. Vercellino and Gabriele Ceccarelli and Francesco Cristofaro and Martina Balli and Federico Bertoglio and G. Bruni and L. Benedetti and Avanzini, {Maria Antonietta} and Marcello Imbriani and Livia Visai",
year = "2016",
month = "7",
day = "1",
doi = "10.3390/nano6070124",
language = "English",
volume = "6",
journal = "Nanomaterials",
issn = "2079-4991",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
number = "7",

}

TY - JOUR

T1 - Nanostructured TiO2 surfaces promote human bone marrow mesenchymal stem cells differentiation to osteoblasts

AU - Vercellino, M.

AU - Ceccarelli, Gabriele

AU - Cristofaro, Francesco

AU - Balli, Martina

AU - Bertoglio, Federico

AU - Bruni, G.

AU - Benedetti, L.

AU - Avanzini, Maria Antonietta

AU - Imbriani, Marcello

AU - Visai, Livia

PY - 2016/7/1

Y1 - 2016/7/1

N2 - Micro-and nano-patterning/modification are emerging strategies to improve surfaces properties that may influence critically cells adherence and differentiation. Aim of this work was to study the in vitro biological reactivity of human bone marrow mesenchymal stem cells (hBMSCs) to a nanostructured titanium dioxide (TiO2) surface in comparison to a coverglass (Glass) in two different culture conditions: with (osteogenic medium (OM)) and without (proliferative medium (PM)) osteogenic factors. To evaluate cell adhesion, hBMSCs phosphorylated focal adhesion kinase (pFAK) foci were analyzed by confocal laser scanning microscopy (CLSM) at 24 h: the TiO2 surface showed a higher number of pFAK foci with respect to Glass. The hBMSCs differentiation to osteoblasts was evaluated in both PM and OM culture conditions by enzyme-linked immunosorbent assay (ELISA), CLSM and real-time quantitative reverse transcription PCR (qRT-PCR) at 28 days. In comparison with Glass, TiO2 surface in combination with OM conditions increased the content of extracellular bone proteins, calcium deposition and alkaline phosphatase activity. The qRT-PCR analysis revealed, both in PM and OM, that TiO2 surface increased at seven and 28 days the expression of osteogenic genes. All together, these results demonstrate the capability of TiO2 nanostructured surface to promote hBMSCs osteoblast differentiation and its potentiality in biomedical applications.

AB - Micro-and nano-patterning/modification are emerging strategies to improve surfaces properties that may influence critically cells adherence and differentiation. Aim of this work was to study the in vitro biological reactivity of human bone marrow mesenchymal stem cells (hBMSCs) to a nanostructured titanium dioxide (TiO2) surface in comparison to a coverglass (Glass) in two different culture conditions: with (osteogenic medium (OM)) and without (proliferative medium (PM)) osteogenic factors. To evaluate cell adhesion, hBMSCs phosphorylated focal adhesion kinase (pFAK) foci were analyzed by confocal laser scanning microscopy (CLSM) at 24 h: the TiO2 surface showed a higher number of pFAK foci with respect to Glass. The hBMSCs differentiation to osteoblasts was evaluated in both PM and OM culture conditions by enzyme-linked immunosorbent assay (ELISA), CLSM and real-time quantitative reverse transcription PCR (qRT-PCR) at 28 days. In comparison with Glass, TiO2 surface in combination with OM conditions increased the content of extracellular bone proteins, calcium deposition and alkaline phosphatase activity. The qRT-PCR analysis revealed, both in PM and OM, that TiO2 surface increased at seven and 28 days the expression of osteogenic genes. All together, these results demonstrate the capability of TiO2 nanostructured surface to promote hBMSCs osteoblast differentiation and its potentiality in biomedical applications.

KW - Biomedical applications

KW - Bone tissue engineering and regeneration

KW - Human bone marrow mesenchymal stem cells

KW - Nano-patterning modification

KW - Titanium dioxide surface

UR - http://www.scopus.com/inward/record.url?scp=84975796428&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84975796428&partnerID=8YFLogxK

U2 - 10.3390/nano6070124

DO - 10.3390/nano6070124

M3 - Article

VL - 6

JO - Nanomaterials

JF - Nanomaterials

SN - 2079-4991

IS - 7

M1 - 124

ER -