Abstract
Micro-and nano-patterning/modification are emerging strategies to improve surfaces properties that may influence critically cells adherence and differentiation. Aim of this work was to study the in vitro biological reactivity of human bone marrow mesenchymal stem cells (hBMSCs) to a nanostructured titanium dioxide (TiO2) surface in comparison to a coverglass (Glass) in two different culture conditions: with (osteogenic medium (OM)) and without (proliferative medium (PM)) osteogenic factors. To evaluate cell adhesion, hBMSCs phosphorylated focal adhesion kinase (pFAK) foci were analyzed by confocal laser scanning microscopy (CLSM) at 24 h: the TiO2 surface showed a higher number of pFAK foci with respect to Glass. The hBMSCs differentiation to osteoblasts was evaluated in both PM and OM culture conditions by enzyme-linked immunosorbent assay (ELISA), CLSM and real-time quantitative reverse transcription PCR (qRT-PCR) at 28 days. In comparison with Glass, TiO2 surface in combination with OM conditions increased the content of extracellular bone proteins, calcium deposition and alkaline phosphatase activity. The qRT-PCR analysis revealed, both in PM and OM, that TiO2 surface increased at seven and 28 days the expression of osteogenic genes. All together, these results demonstrate the capability of TiO2 nanostructured surface to promote hBMSCs osteoblast differentiation and its potentiality in biomedical applications.
| Original language | English |
|---|---|
| Article number | 124 |
| Journal | Nanomaterials |
| Volume | 6 |
| Issue number | 7 |
| DOIs | |
| Publication status | Published - Jul 1 2016 |
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Keywords
- Biomedical applications
- Bone tissue engineering and regeneration
- Human bone marrow mesenchymal stem cells
- Nano-patterning modification
- Titanium dioxide surface
ASJC Scopus subject areas
- Materials Science(all)
- Chemical Engineering(all)
Cite this
Nanostructured TiO2 surfaces promote human bone marrow mesenchymal stem cells differentiation to osteoblasts. / Vercellino, M.; Ceccarelli, Gabriele; Cristofaro, Francesco; Balli, Martina; Bertoglio, Federico; Bruni, G.; Benedetti, L.; Avanzini, Maria Antonietta; Imbriani, Marcello; Visai, Livia.
In: Nanomaterials, Vol. 6, No. 7, 124, 01.07.2016.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Nanostructured TiO2 surfaces promote human bone marrow mesenchymal stem cells differentiation to osteoblasts
AU - Vercellino, M.
AU - Ceccarelli, Gabriele
AU - Cristofaro, Francesco
AU - Balli, Martina
AU - Bertoglio, Federico
AU - Bruni, G.
AU - Benedetti, L.
AU - Avanzini, Maria Antonietta
AU - Imbriani, Marcello
AU - Visai, Livia
PY - 2016/7/1
Y1 - 2016/7/1
N2 - Micro-and nano-patterning/modification are emerging strategies to improve surfaces properties that may influence critically cells adherence and differentiation. Aim of this work was to study the in vitro biological reactivity of human bone marrow mesenchymal stem cells (hBMSCs) to a nanostructured titanium dioxide (TiO2) surface in comparison to a coverglass (Glass) in two different culture conditions: with (osteogenic medium (OM)) and without (proliferative medium (PM)) osteogenic factors. To evaluate cell adhesion, hBMSCs phosphorylated focal adhesion kinase (pFAK) foci were analyzed by confocal laser scanning microscopy (CLSM) at 24 h: the TiO2 surface showed a higher number of pFAK foci with respect to Glass. The hBMSCs differentiation to osteoblasts was evaluated in both PM and OM culture conditions by enzyme-linked immunosorbent assay (ELISA), CLSM and real-time quantitative reverse transcription PCR (qRT-PCR) at 28 days. In comparison with Glass, TiO2 surface in combination with OM conditions increased the content of extracellular bone proteins, calcium deposition and alkaline phosphatase activity. The qRT-PCR analysis revealed, both in PM and OM, that TiO2 surface increased at seven and 28 days the expression of osteogenic genes. All together, these results demonstrate the capability of TiO2 nanostructured surface to promote hBMSCs osteoblast differentiation and its potentiality in biomedical applications.
AB - Micro-and nano-patterning/modification are emerging strategies to improve surfaces properties that may influence critically cells adherence and differentiation. Aim of this work was to study the in vitro biological reactivity of human bone marrow mesenchymal stem cells (hBMSCs) to a nanostructured titanium dioxide (TiO2) surface in comparison to a coverglass (Glass) in two different culture conditions: with (osteogenic medium (OM)) and without (proliferative medium (PM)) osteogenic factors. To evaluate cell adhesion, hBMSCs phosphorylated focal adhesion kinase (pFAK) foci were analyzed by confocal laser scanning microscopy (CLSM) at 24 h: the TiO2 surface showed a higher number of pFAK foci with respect to Glass. The hBMSCs differentiation to osteoblasts was evaluated in both PM and OM culture conditions by enzyme-linked immunosorbent assay (ELISA), CLSM and real-time quantitative reverse transcription PCR (qRT-PCR) at 28 days. In comparison with Glass, TiO2 surface in combination with OM conditions increased the content of extracellular bone proteins, calcium deposition and alkaline phosphatase activity. The qRT-PCR analysis revealed, both in PM and OM, that TiO2 surface increased at seven and 28 days the expression of osteogenic genes. All together, these results demonstrate the capability of TiO2 nanostructured surface to promote hBMSCs osteoblast differentiation and its potentiality in biomedical applications.
KW - Biomedical applications
KW - Bone tissue engineering and regeneration
KW - Human bone marrow mesenchymal stem cells
KW - Nano-patterning modification
KW - Titanium dioxide surface
UR - http://www.scopus.com/inward/record.url?scp=84975796428&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84975796428&partnerID=8YFLogxK
U2 - 10.3390/nano6070124
DO - 10.3390/nano6070124
M3 - Article
VL - 6
JO - Nanomaterials
JF - Nanomaterials
SN - 2079-4991
IS - 7
M1 - 124
ER -