Nasal cell DNA methylation, inflammation, lung function and wheezing in children with asthma

Andrea Baccarelli, Franca Rusconi, Valentina Bollati, Dolores Catelan, Gabriele Accetta, Lifang Hou, Fabio Barbone, Pier Alberto Bertazzi, Annibale Biggeri

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Aims: DNA methylation is increasingly proposed as a mechanism for underlying asthma-related inflammation. However, epigenetic studies are constrained by uncertainties on whether samples that can be easily collected in human individuals can provide informative results. Methods: Two nasal cell DNA samples were collected on different days by nasal brushings from 35 asthmatic children aged between 8 and 11 years old. We correlated DNA methylation of IL-6, iNOS, Alu and LINE-1 with fractional exhaled nitric oxide, forced expiratory volume in 1 s and wheezing. Results: Fractional exhaled nitric oxide increased in association with lower promoter methylation of both IL-6 (+29.0%; p = 0.004) and iNOS (+41.0%; p = 0.002). Lower IL-6 methylation was nonsignificantly associated with wheezing during the week of the study (odds ratio = 2.3; p = 0.063). Conclusion: Our findings support the use of nasal cell DNA for human epigenetic studies of asthma.

Original languageEnglish
Pages (from-to)91-100
Number of pages10
JournalEpigenomics
Volume4
Issue number1
DOIs
Publication statusPublished - Feb 2012

Fingerprint

Respiratory Sounds
DNA Methylation
Nose
Interleukin-6
Pneumonia
Asthma
Epigenomics
Methylation
Nitric Oxide
DNA
Forced Expiratory Volume
Uncertainty
Odds Ratio
Inflammation

Keywords

  • airway obstruction
  • asthma
  • children
  • DNA methylation
  • epigenetics
  • inflammation

ASJC Scopus subject areas

  • Genetics
  • Cancer Research

Cite this

Baccarelli, A., Rusconi, F., Bollati, V., Catelan, D., Accetta, G., Hou, L., ... Biggeri, A. (2012). Nasal cell DNA methylation, inflammation, lung function and wheezing in children with asthma. Epigenomics, 4(1), 91-100. https://doi.org/10.2217/epi.11.106

Nasal cell DNA methylation, inflammation, lung function and wheezing in children with asthma. / Baccarelli, Andrea; Rusconi, Franca; Bollati, Valentina; Catelan, Dolores; Accetta, Gabriele; Hou, Lifang; Barbone, Fabio; Bertazzi, Pier Alberto; Biggeri, Annibale.

In: Epigenomics, Vol. 4, No. 1, 02.2012, p. 91-100.

Research output: Contribution to journalArticle

Baccarelli, A, Rusconi, F, Bollati, V, Catelan, D, Accetta, G, Hou, L, Barbone, F, Bertazzi, PA & Biggeri, A 2012, 'Nasal cell DNA methylation, inflammation, lung function and wheezing in children with asthma', Epigenomics, vol. 4, no. 1, pp. 91-100. https://doi.org/10.2217/epi.11.106
Baccarelli A, Rusconi F, Bollati V, Catelan D, Accetta G, Hou L et al. Nasal cell DNA methylation, inflammation, lung function and wheezing in children with asthma. Epigenomics. 2012 Feb;4(1):91-100. https://doi.org/10.2217/epi.11.106
Baccarelli, Andrea ; Rusconi, Franca ; Bollati, Valentina ; Catelan, Dolores ; Accetta, Gabriele ; Hou, Lifang ; Barbone, Fabio ; Bertazzi, Pier Alberto ; Biggeri, Annibale. / Nasal cell DNA methylation, inflammation, lung function and wheezing in children with asthma. In: Epigenomics. 2012 ; Vol. 4, No. 1. pp. 91-100.
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