TY - JOUR
T1 - Nasal microbiota modifies the effects of particulate air pollution on plasma extracellular vesicles
AU - Mariani, Jacopo
AU - Favero, Chiara
AU - Carugno, Michele
AU - Pergoli, Laura
AU - Ferrari, Luca
AU - Bonzini, Matteo
AU - Cattaneo, Andrea
AU - Pesatori, Angela Cecilia
AU - Bollati, Valentina
PY - 2020/1/2
Y1 - 2020/1/2
N2 - Air pollution exposure has been linked to modifications of both extracellular vesicle (EV) concentration and nasal microbiota structure (NMB), which might act as the respiratory health gatekeeper. This study aimed to assess whether an unbalanced NMB could modify the effect of particulate matter (PM) exposure on plasmatic EV levels. Due to two different NMB taxonomical profiles characterized by a widely different relative abundance of the Moraxella genus, the enrolled population was stratified into Mor‐ (balanced NMB) and Mor+ (unbalanced NMB) groups (Moraxella genus’s cut‐off ≤25% and >25%, respectively). EV features were assessed by nanoparticle tracking analysis (NTA) and flow‐cytometry (FC). Multivariable analyses were applied on EV outcomes to evaluate a possible association between PM10 and PM2.5 and plasmatic EV levels. The Mor‐ group revealed positive associations between PM levels and plasmatic CD105+ EVs (GMR = 4.39 p = 0.02) as for total EV count (GMR = 1.92 p = 0.02). Conversely, the Mor+ group showed a negative association between exposure and EV outcomes (CD66+ GMR = 0.004 p = 0.01; EpCAM+ GMR = 0.005 p = 0.01). Our findings provide an insight regarding how a balanced NMB may help to counteract PM exposure effects in terms of plasmatic EV concentration. Further research is necessary to understand the relationship between the host and the NMB to disentangle the mechanism exerted by inhaled pollutants in modulating EVs and NMB.
AB - Air pollution exposure has been linked to modifications of both extracellular vesicle (EV) concentration and nasal microbiota structure (NMB), which might act as the respiratory health gatekeeper. This study aimed to assess whether an unbalanced NMB could modify the effect of particulate matter (PM) exposure on plasmatic EV levels. Due to two different NMB taxonomical profiles characterized by a widely different relative abundance of the Moraxella genus, the enrolled population was stratified into Mor‐ (balanced NMB) and Mor+ (unbalanced NMB) groups (Moraxella genus’s cut‐off ≤25% and >25%, respectively). EV features were assessed by nanoparticle tracking analysis (NTA) and flow‐cytometry (FC). Multivariable analyses were applied on EV outcomes to evaluate a possible association between PM10 and PM2.5 and plasmatic EV levels. The Mor‐ group revealed positive associations between PM levels and plasmatic CD105+ EVs (GMR = 4.39 p = 0.02) as for total EV count (GMR = 1.92 p = 0.02). Conversely, the Mor+ group showed a negative association between exposure and EV outcomes (CD66+ GMR = 0.004 p = 0.01; EpCAM+ GMR = 0.005 p = 0.01). Our findings provide an insight regarding how a balanced NMB may help to counteract PM exposure effects in terms of plasmatic EV concentration. Further research is necessary to understand the relationship between the host and the NMB to disentangle the mechanism exerted by inhaled pollutants in modulating EVs and NMB.
KW - 16S
KW - Bacteria
KW - Dysbiosis
KW - Extracellular vesicles
KW - Flow‐cytometry
KW - Microbiome
KW - Moraxella
KW - Nanoparticle tracking analysis
KW - Nasal microbiota
KW - Particulate matter
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UR - http://www.scopus.com/inward/citedby.url?scp=85078255802&partnerID=8YFLogxK
U2 - 10.3390/ijerph17020611
DO - 10.3390/ijerph17020611
M3 - Article
C2 - 31963616
AN - SCOPUS:85078255802
VL - 17
JO - International Journal of Environmental Research and Public Health
JF - International Journal of Environmental Research and Public Health
SN - 1661-7827
IS - 2
M1 - 611
ER -