Nasal neutrophilia and release of myeloperoxidase induced by nasal challenge with platelet activating factor: Different degrees of responsiveness in atopic and nonatopic subjects

A. Miadonna, N. Milazzo, M. Lorini, A. Sala, A. Tedeschi

Research output: Contribution to journalArticle

Abstract

Background: Nasal challenge with platelet activating factor (PAF) is able to induce local neurophilia, with a different degree of responsiveness in atopic subjects and in nonatopic subjects. We investigated whether nasal accumulation of neutrophils induced by PAF is accompanied by the release of neutrophil-derived mediators. Methods: Nasal lavages were performed before and after challenge with PAF (500 nmol), lyso-PAF (500 nmol), and saline solution in 10 patients with allergic rhinitis and 10 normal subjects to evaluate changes in neutrophil counts and the release of myeloperoxidase (MPO) and immunoreactive leukotriene B4. Results: PAF caused neutrophilia, which appeared after 30 minutes in atopic subjects and after 3 hours in nonatopic subjects. Furthermore, when compared with saline insufflation, PAF caused a significant release of MPO in the nasal lavage fluids collected 30 minutes, 3 hours, and 24 hours after challenge in atopic subjects and 3 hours after challenge in nonatopic subjects, with higher values in the former than in the latter. Neutrophil counts correlated with MPO levels in the nasal lavages collected after PAF challenge. A lower degree of neutrophilia was found 3 hours after stimulation with lyso-PAF in both groups of subjects, with a marginal release of MPO in atopic subjects only. No significant increase of immunoreactive leukotriene B4 levels in nasal lavages was found after challenge with either PAF or lyso-PAF. Conclusion: These results indicate that PAF-induced neutrophilia in the nose is accompanied by the release of MPO, which appears earlier and is more marked in atopic subjects than in nonatopic subjects.

Original languageEnglish
Pages (from-to)947-954
Number of pages8
JournalJournal of Allergy and Clinical Immunology
Volume97
Issue number4
DOIs
Publication statusPublished - 1996

Fingerprint

Platelet Activating Factor
Nose
Peroxidase
Nasal Lavage
Neutrophils
Leukotriene B4
Nasal Lavage Fluid
Insufflation
Sodium Chloride

Keywords

  • Leukotriene B
  • lyso-PAF
  • myeloperoxidase
  • nasal lavage
  • neutrophil
  • PAF

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Nasal neutrophilia and release of myeloperoxidase induced by nasal challenge with platelet activating factor : Different degrees of responsiveness in atopic and nonatopic subjects. / Miadonna, A.; Milazzo, N.; Lorini, M.; Sala, A.; Tedeschi, A.

In: Journal of Allergy and Clinical Immunology, Vol. 97, No. 4, 1996, p. 947-954.

Research output: Contribution to journalArticle

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abstract = "Background: Nasal challenge with platelet activating factor (PAF) is able to induce local neurophilia, with a different degree of responsiveness in atopic subjects and in nonatopic subjects. We investigated whether nasal accumulation of neutrophils induced by PAF is accompanied by the release of neutrophil-derived mediators. Methods: Nasal lavages were performed before and after challenge with PAF (500 nmol), lyso-PAF (500 nmol), and saline solution in 10 patients with allergic rhinitis and 10 normal subjects to evaluate changes in neutrophil counts and the release of myeloperoxidase (MPO) and immunoreactive leukotriene B4. Results: PAF caused neutrophilia, which appeared after 30 minutes in atopic subjects and after 3 hours in nonatopic subjects. Furthermore, when compared with saline insufflation, PAF caused a significant release of MPO in the nasal lavage fluids collected 30 minutes, 3 hours, and 24 hours after challenge in atopic subjects and 3 hours after challenge in nonatopic subjects, with higher values in the former than in the latter. Neutrophil counts correlated with MPO levels in the nasal lavages collected after PAF challenge. A lower degree of neutrophilia was found 3 hours after stimulation with lyso-PAF in both groups of subjects, with a marginal release of MPO in atopic subjects only. No significant increase of immunoreactive leukotriene B4 levels in nasal lavages was found after challenge with either PAF or lyso-PAF. Conclusion: These results indicate that PAF-induced neutrophilia in the nose is accompanied by the release of MPO, which appears earlier and is more marked in atopic subjects than in nonatopic subjects.",
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