Method-dependent variability of enzyme catalytic activities jeopardizes the use of uniform clinical decision limits and confuses the understanding of results observed in patient sera and in control materials as well. Results by different methods may be harmonized making the results they produce traceable to a selected higher metrological level: however, it may be easier to achieve result traceability by widespread use of one selected analytical principle comparable with a reference, i.e. the IFCC, reference procedure. The aim of this work was to check the spread in Italian laboratories of assays based on IFCC reference method principles for measurement of alanine transaminase (ALT), α-amylase (AMY), aspartate transaminase (AST), creatine kinase (CK), γ-glutamiltransferase (GGT), and lactate dehydrogenase (LDH) activities. By distributing a questionnaire to organizers of five national EQAS, we collected information about the enzyme analytical methodologies selected in about 1400 laboratories. We found that 86%, 44%, 15% and 10% of laboratories adopted the relevant IFCC principle for CK, AMY, LDH and AST/ALT measurements, respectively. For GGT we observed that the IFCC principle was adopted by 56% of laboratories participating in the two EQAS distinguishing a specific group for the IFCC method. However, 83% of the laboratories participating in EQAS used L-γ-glutamyl-3-carboxy-4-nitroanilide as substrate.
|Translated title of the contribution||National survey on the use of commercial assays employing method principles in agreement with IFCC reference methods for the measurement of catalytic activity of serum enzymes|
|Number of pages||5|
|Publication status||Published - Apr 2010|
ASJC Scopus subject areas
- Clinical Biochemistry
- Biochemistry, medical
- Medical Laboratory Technology