Natural antibodies to tumor-associated antigens

Sheila María Álvarez-Fernández; Lucia De Monte; Massimo Alessio

doi:10.1007/978-1-4939-3338-9_2

Natural antibodies to tumor-associated antigens

Sheila María Álvarez-Fernández, Lucia De Monte, Massimo Alessio

IRCCS Ospedale San Raffaele

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

Natural autoantibodies raised by humoral immune response to cancer can be exploited to identify potential tumor-associated antigens (TAAs), and might constitute new putative prognostic and/or diagnostic biomarkers. Here we describe how sera from tumor patients can be used to identify TAAs by screening antibody immunoreactivity against the cancer proteome resolved by two-dimensional gel electrophoresis.

Original language	English
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	11-25
Number of pages	15
Volume	1393
DOIs	https://doi.org/10.1007/978-1-4939-3338-9_2
Publication status	Published - Apr 1 2016

Publication series

Name	Methods in Molecular Biology
Volume	1393
ISSN (Print)	10643745

Keywords

2D-electrophoresis
Autoantibodies
Immunoproteomics
Tumor-associated antigens
Western blot

ASJC Scopus subject areas

Molecular Biology
Genetics

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10.1007/978-1-4939-3338-9_2

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Álvarez-Fernández, S. M., De Monte, L., & Alessio, M. (2016). Natural antibodies to tumor-associated antigens. In Methods in Molecular Biology (Vol. 1393, pp. 11-25). (Methods in Molecular Biology; Vol. 1393). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-3338-9_2

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