Natural antibodies to tumor-associated antigens

Sheila María Álvarez-Fernández, Lucia De Monte, Massimo Alessio

Research output: Chapter in Book/Report/Conference proceedingChapter


Natural autoantibodies raised by humoral immune response to cancer can be exploited to identify potential tumor-associated antigens (TAAs), and might constitute new putative prognostic and/or diagnostic biomarkers. Here we describe how sera from tumor patients can be used to identify TAAs by screening antibody immunoreactivity against the cancer proteome resolved by two-dimensional gel electrophoresis.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages15
Publication statusPublished - Apr 1 2016

Publication series

NameMethods in Molecular Biology
ISSN (Print)10643745


  • 2D-electrophoresis
  • Autoantibodies
  • Immunoproteomics
  • Tumor-associated antigens
  • Western blot

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Álvarez-Fernández, S. M., De Monte, L., & Alessio, M. (2016). Natural antibodies to tumor-associated antigens. In Methods in Molecular Biology (Vol. 1393, pp. 11-25). (Methods in Molecular Biology; Vol. 1393). Humana Press Inc..