TY - JOUR
T1 - Natural compounds against neurodegenerative diseases
T2 - molecular characterization of the interaction of catechins from green tea with Aβ1-42, PrP106-126, and ataxin-3 oligomers
AU - Sironi, Erika
AU - Colombo, Laura
AU - Lompo, Angela
AU - Messa, Massimo
AU - Bonanomi, Marcella
AU - Regonesi, Maria E lena
AU - Salmona, Mario
AU - Airoldi, Cristina
PY - 2014/10/13
Y1 - 2014/10/13
N2 - By combining NMR spectroscopy, transmission electron microscopy, and circular dichroism we have identified the structural determinants involved in the interaction of green tea catechins with Aβ1-42, PrP106-126, and ataxin-3 oligomers. The data allow the elucidation of their mechanism of action, showing that the flavan-3-ol unit of catechins is essential for interaction. At the same time, the gallate moiety, when present, seems to increase the affinity for the target proteins. These results provide important information for the rational design of new compounds with anti-amyloidogenic activity and/or molecular tools for the specific targeting of amyloid aggregates in vivo.
AB - By combining NMR spectroscopy, transmission electron microscopy, and circular dichroism we have identified the structural determinants involved in the interaction of green tea catechins with Aβ1-42, PrP106-126, and ataxin-3 oligomers. The data allow the elucidation of their mechanism of action, showing that the flavan-3-ol unit of catechins is essential for interaction. At the same time, the gallate moiety, when present, seems to increase the affinity for the target proteins. These results provide important information for the rational design of new compounds with anti-amyloidogenic activity and/or molecular tools for the specific targeting of amyloid aggregates in vivo.
KW - amyloid peptides
KW - circular dichroism
KW - molecular recognition
KW - NMR spectroscopy
KW - structure-activity relationship
UR - http://www.scopus.com/inward/record.url?scp=84946168607&partnerID=8YFLogxK
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U2 - 10.1002/chem.201403188
DO - 10.1002/chem.201403188
M3 - Article
C2 - 25179684
AN - SCOPUS:84942077065
VL - 20
SP - 13793
EP - 13800
JO - Chemistry - A European Journal
JF - Chemistry - A European Journal
SN - 0947-6539
IS - 42
ER -