Natural Killer (NK)/melanoma cell interaction induces NK-mediated release of chemotactic High Mobility Group Box-1 (HMGB1) capable of amplifying NK cell recruitment

Monica Parodi, Marco Pedrazzi, Claudia Cantoni, Monica Averna, Mauro Patrone, Maria Cavaletto, Stefano Spertino, Daniela Pende, Mirna Balsamo, Gabriella Pietra, Simona Sivori, Simona Carlomagno, Maria Cristina Mingari, Lorenzo Moretta, Bianca Sparatore, Massimo Vitale

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

In this study we characterize a new mechanism by which Natural Killer (NK) cells may amplify their recruitment to tumors. We show that NK cells, upon interaction with melanoma cells, can release a chemotactic form of High Mobility Group Box-1 (HMGB1) protein capable of attracting additional activated NK cells. We first demonstrate that the engagement of different activating NK cell receptors, including those mainly involved in tumor cell recognition can induce the active release of HMGB1. Then we show that during NK-mediated tumor cell killing two HMGB1 forms are released, each displaying a specific electrophoretic mobility possibly corresponding to a different redox status. By the comparison of normal and perforin-defective NK cells (which are unable to kill target cells) we demonstrate that, in NK/melanoma cell co-cultures, NK cells specifically release an HMGB1 form that acts as chemoattractant, while dying tumor cells passively release a non-chemotactic HMGB1. Finally, we show that Receptor for Advanced Glycation End products is expressed by NK cells and mediates HMGB1-induced NK cell chemotaxis. Proteomic analysis of NK cells exposed to recombinant HMGB1 revealed that this molecule, besides inducing immediate chemotaxis, also promotes changes in the expression of proteins involved in the regulation of the cytoskeletal network. Importantly, these modifications could be associated with an increased motility of NK cells. Thus, our findings allow the definition of a previously unidentified mechanism used by NK cells to amplify their response to tumors, and provide additional clues for the emerging role of HMGB1 in immunomodulation and tumor immunity.

Original languageEnglish
JournalOncoImmunology
Volume4
Issue number12
DOIs
Publication statusPublished - Dec 2 2015

Fingerprint

Cell Communication
Natural Killer Cells
Melanoma
Neoplasms
Chemotaxis
Natural Killer Cell Receptors
HMGB1 Protein
Perforin
Immunomodulation
Chemotactic Factors
Coculture Techniques
Proteomics
Oxidation-Reduction
Immunity
Cell Culture Techniques

Keywords

  • chemotaxis
  • HMGB1
  • melanoma cells
  • NK cells
  • tumor microenvironment

ASJC Scopus subject areas

  • Immunology and Allergy
  • Oncology
  • Immunology

Cite this

Natural Killer (NK)/melanoma cell interaction induces NK-mediated release of chemotactic High Mobility Group Box-1 (HMGB1) capable of amplifying NK cell recruitment. / Parodi, Monica; Pedrazzi, Marco; Cantoni, Claudia; Averna, Monica; Patrone, Mauro; Cavaletto, Maria; Spertino, Stefano; Pende, Daniela; Balsamo, Mirna; Pietra, Gabriella; Sivori, Simona; Carlomagno, Simona; Mingari, Maria Cristina; Moretta, Lorenzo; Sparatore, Bianca; Vitale, Massimo.

In: OncoImmunology, Vol. 4, No. 12, 02.12.2015.

Research output: Contribution to journalArticle

Parodi, Monica ; Pedrazzi, Marco ; Cantoni, Claudia ; Averna, Monica ; Patrone, Mauro ; Cavaletto, Maria ; Spertino, Stefano ; Pende, Daniela ; Balsamo, Mirna ; Pietra, Gabriella ; Sivori, Simona ; Carlomagno, Simona ; Mingari, Maria Cristina ; Moretta, Lorenzo ; Sparatore, Bianca ; Vitale, Massimo. / Natural Killer (NK)/melanoma cell interaction induces NK-mediated release of chemotactic High Mobility Group Box-1 (HMGB1) capable of amplifying NK cell recruitment. In: OncoImmunology. 2015 ; Vol. 4, No. 12.
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abstract = "In this study we characterize a new mechanism by which Natural Killer (NK) cells may amplify their recruitment to tumors. We show that NK cells, upon interaction with melanoma cells, can release a chemotactic form of High Mobility Group Box-1 (HMGB1) protein capable of attracting additional activated NK cells. We first demonstrate that the engagement of different activating NK cell receptors, including those mainly involved in tumor cell recognition can induce the active release of HMGB1. Then we show that during NK-mediated tumor cell killing two HMGB1 forms are released, each displaying a specific electrophoretic mobility possibly corresponding to a different redox status. By the comparison of normal and perforin-defective NK cells (which are unable to kill target cells) we demonstrate that, in NK/melanoma cell co-cultures, NK cells specifically release an HMGB1 form that acts as chemoattractant, while dying tumor cells passively release a non-chemotactic HMGB1. Finally, we show that Receptor for Advanced Glycation End products is expressed by NK cells and mediates HMGB1-induced NK cell chemotaxis. Proteomic analysis of NK cells exposed to recombinant HMGB1 revealed that this molecule, besides inducing immediate chemotaxis, also promotes changes in the expression of proteins involved in the regulation of the cytoskeletal network. Importantly, these modifications could be associated with an increased motility of NK cells. Thus, our findings allow the definition of a previously unidentified mechanism used by NK cells to amplify their response to tumors, and provide additional clues for the emerging role of HMGB1 in immunomodulation and tumor immunity.",
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AU - Patrone, Mauro

AU - Cavaletto, Maria

AU - Spertino, Stefano

AU - Pende, Daniela

AU - Balsamo, Mirna

AU - Pietra, Gabriella

AU - Sivori, Simona

AU - Carlomagno, Simona

AU - Mingari, Maria Cristina

AU - Moretta, Lorenzo

AU - Sparatore, Bianca

AU - Vitale, Massimo

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