Natural polymorphisms of HIV-1 subtype-C integrase coding region in a large group of ARV-naïve infected individuals

S. Dimonte, M. Babakir-Mina, S. Aquaro, C. F. Perno

Research output: Contribution to journalArticle

Abstract

Purpose: Integrase (IN) is an enzyme produced by human immunodeficiency virus (HIV)-1 that enables its genetic material to be integrated into the DNA of the infected cell. Still now, few data are available with detailed analysis of the natural IN polymorphisms of HIV-1 subtype-C in datasets retrieved from antiretroviral-naïve patients; this study focuses on these polymorphisms. Methods: The analysis included 335 HIV-1 subtype-C IN sequences (one per patient). Multi-alignment of IN sequences was performed, and for the definition of a polymorphism, only amino acid changes with prevalence ≥3 % among treatment-naïve patients were considered. Results: Seventy IN amino acid positions were fully conserved. Differently, forty-six IN amino acid polymorphic positions were observed, 12 within the N-terminal domain and 13 within the C-terminal domain. In the DDE-catalytic motif, only one mutation per site (D64G/D116G/E152K) was found, while a low variability (

Original languageEnglish
Pages (from-to)1097-1102
Number of pages6
JournalInfection
Volume41
Issue number6
DOIs
Publication statusPublished - Dec 2013

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Keywords

  • Drug resistance
  • HIV
  • Integrase inhibitors
  • Polymorphism
  • Prevalence
  • Subtype-C

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

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