NCAM1 is the Target of miRNA-572: Validation in the Human Oligodendroglial Cell Line

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Abstract

The neural cell adhesion molecule 1 (NCAM1) is a fundamental protein in cell–cell interaction and in cellular developmental processes, and its dysregulation is involved in a number of diseases including multiple sclerosis. Studies in rats suggest that the modulation of NCAM1 expression is regulated by miRNA-572, but no data are available confirming such interaction in the human system. We analyzed whether this is the case using a human oligodendroglial cell line (MO3.13). MO3.13 cells were transfected with miRNA-572 mimic and inhibitor separately; NCAM1 mRNA and protein expression levels were analyzed at different time points after transfection. Results indicated that NCAM1 expression is increased after transfection with miRNA-572 inhibitor, whereas it is decreased after transfection with the mimic (p < 0.005). The interaction between NCAM1 and miRNA-572 was subsequently confirmed in a Vero cell line that does not express NCAM1, by luciferase assay after transfection with NCAM1. These results confirm that miRNA-572 regulates NCAM1 and for the first time demonstrate that this interaction regulates NCAM1 expression in human cells. Data herein also support the hypothesis that miRNA-572 is involved in diseases associated with NCAM1 deregulation, suggesting its possible use as a biomarker in these diseases.

Original languageEnglish
Pages (from-to)1-10
Number of pages10
JournalCellular and Molecular Neurobiology
DOIs
Publication statusE-pub ahead of print - Mar 22 2017

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Keywords

  • Human oligodendrocytes
  • microRNA
  • Multiple sclerosis
  • Myelination
  • Neuronal cell adhesion molecule
  • Transfection

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Cell Biology

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