Necdin mediates skeletal muscle regeneration by promoting myoblast survival and differentiation

Daniela Deponti, Stéphanie François, Silvia Baesso, Clara Sciorati, Anna Innocenzi, Vania Broccoli, Françoise Muscatelli, Raffaella Meneveri, Emilio Clementi, Giulio Cossu, Silvia Brunelli

Research output: Contribution to journalArticlepeer-review

Abstract

Regeneration of muscle fibers that are lost during pathological muscle degeneration or after injuries is sustained by the production of new myofibers. An important cell type involved in muscle regeneration is the satellite cell. Necdin is a protein expressed in satellite cell-derived myogenic precursors during perinatal growth. However, its function in myogenesis is not known. We compare transgenic mice that overexpress necdin in skeletal muscle with both wild-type and necdin null mice. After muscle injury the necdin null mice show a considerable defect in muscle healing, whereas mice that overexpress necdin show a substantial increase in myofiber regeneration. We also find that in muscle, necdin increases myogenin expression, accelerates differentiation, and counteracts myoblast apoptosis. Collectively, these data clarify the function and mechanism of necdin in skeletal muscle and show the importance of necdin in muscle regeneration.

Original languageEnglish
Pages (from-to)305-319
Number of pages15
JournalJournal of Cell Biology
Volume179
Issue number2
DOIs
Publication statusPublished - Oct 22 2007

ASJC Scopus subject areas

  • Cell Biology

Fingerprint

Dive into the research topics of 'Necdin mediates skeletal muscle regeneration by promoting myoblast survival and differentiation'. Together they form a unique fingerprint.

Cite this