Neocortical and hippocampal changes after multiple pilocarpine-induced status epilepticus in rats

Alexandre Valotta Da Silva, Maria Cristina Regondi, Barbara Cipelletti, Carolina Frassoni, Esper Abrão Cavalheiro, Roberto Spreafico

Research output: Contribution to journalArticle

Abstract

Purpose: Multiple episodes of pilocarpine-induced status epilepticus (SE) in developing rats (P7-P9) lead to progressive epileptiform activity and severe cognitive impairment in adulthood. The present work studied possible underlying abnormalities in the neocortex and hippocampus of pilocarpine-treated animals. Methods: Wistar rats were submitted to pilocarpine-induced SE at P7, P8, and P9, and were killed at P35. Immunocytochemistry was performed on 50-μm vibratome sections, by using antibodies against nonphosphorylated neurofilament (SMI-311), parvalbumin (PV), calbindin (CB), calretinin (CR), and glutamate decarboxylase (GAD-65). Ten-micron cryostat sections were processed for immunohistoblot by using antibodies against GluR1, GluR2/3, and GluR4 α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor subunits and NR2ab N-methyl-D-aspartate (NMDA) receptor subunit. Results: Adult rats submitted to SE at P7-9 showed: (a) altered distribution of neocortical interneurons; (b) increased cortical and reduced hippocampal GAD-65 expression; and (c) altered expression of hippocampal AMPA and NMDA receptors. Conclusions: We conclude that multiple SE episodes during P7-9 generate long-lasting disturbances that underlie behavioral and electrographic abnormalities later in life.

Original languageEnglish
Pages (from-to)636-642
Number of pages7
JournalEpilepsia
Volume46
Issue number5
DOIs
Publication statusPublished - May 2005

Fingerprint

Pilocarpine
Status Epilepticus
N-Methyl-D-Aspartate Receptors
Calbindin 2
Calbindins
Parvalbumins
alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid
Glutamate Decarboxylase
AMPA Receptors
Intermediate Filaments
Antibodies
Neocortex
Interneurons
Wistar Rats
Hippocampus
Immunohistochemistry
Acids

Keywords

  • Early insult
  • Immature brain
  • Immunocytochemistry
  • Immunohistoblot

ASJC Scopus subject areas

  • Clinical Neurology
  • Neuroscience(all)

Cite this

Neocortical and hippocampal changes after multiple pilocarpine-induced status epilepticus in rats. / Da Silva, Alexandre Valotta; Regondi, Maria Cristina; Cipelletti, Barbara; Frassoni, Carolina; Abrão Cavalheiro, Esper; Spreafico, Roberto.

In: Epilepsia, Vol. 46, No. 5, 05.2005, p. 636-642.

Research output: Contribution to journalArticle

Da Silva, Alexandre Valotta ; Regondi, Maria Cristina ; Cipelletti, Barbara ; Frassoni, Carolina ; Abrão Cavalheiro, Esper ; Spreafico, Roberto. / Neocortical and hippocampal changes after multiple pilocarpine-induced status epilepticus in rats. In: Epilepsia. 2005 ; Vol. 46, No. 5. pp. 636-642.
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