Neoplastic transformation of mast cells by Abelson-MuLV: abrogation of IL-3 dependence by a nonautocrine mechanism

Jacalyn H. Pierce, Pier Paolo Di Fiore, Stuart A. Aaronson, Michael Potter, Janet Pumphrey, Allen Scott, James N. Ihle

Research output: Contribution to journalArticle

Abstract

Normal mast cells can be propagated in culture when medium is supplemented with interleukin-3 (IL-3). We demonstrate that Abelson-MuLV (Ab-MuLV) infection of mast cells eliminates dependence on IL-3 for growth. By contrast, Harvey, BALB, and Moloney MSV, which also productively infect mast cells, are unable to relieve IL-3 dependence. Ab-MuLV induced IL-3-independent lines express the v-abl-specific transforming protein and have phenotypic characteristics of mast cells. These cells also possess high loning efficiencies in soft agarose and are tumorigenic in nude mice. In addition, Ab-MuLV induces transplantable mastocytomas in pristane-primed adult mice resistant to lymphoid transformation, defining a new hematopoietic target for malignant transformation by this virus. None of the Ab-MuLV-derived transformants express or secrete detectable levels of IL-3 nor is their growth inhibited by anti-IL-3 serum. These results argue that Ab-MuLV abrogation of the IL-3 requirement is not due to an autocrine mechanism.

Original languageEnglish
Pages (from-to)685-693
Number of pages9
JournalCell
Volume41
Issue number3
DOIs
Publication statusPublished - 1985

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Interleukin-3
Mast Cells
Mastocytoma
Growth
Viruses
Nude Mice
Sepharose
Culture Media
Infection
Serum
Proteins

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Medicine(all)

Cite this

Neoplastic transformation of mast cells by Abelson-MuLV : abrogation of IL-3 dependence by a nonautocrine mechanism. / Pierce, Jacalyn H.; Paolo Di Fiore, Pier; Aaronson, Stuart A.; Potter, Michael; Pumphrey, Janet; Scott, Allen; Ihle, James N.

In: Cell, Vol. 41, No. 3, 1985, p. 685-693.

Research output: Contribution to journalArticle

Pierce, Jacalyn H. ; Paolo Di Fiore, Pier ; Aaronson, Stuart A. ; Potter, Michael ; Pumphrey, Janet ; Scott, Allen ; Ihle, James N. / Neoplastic transformation of mast cells by Abelson-MuLV : abrogation of IL-3 dependence by a nonautocrine mechanism. In: Cell. 1985 ; Vol. 41, No. 3. pp. 685-693.
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