TY - JOUR
T1 - Nerve growth factor is an autocrine survival factor for memory B lymphocytes
AU - Torcia, Maria
AU - Bracci-Laudiero, Luisa
AU - Lucibello, Maria
AU - Nencioni, Lucia
AU - Labardi, Danilo
AU - Rubartelli, Anna
AU - Cozzolino, Federico
AU - Aloe, Luigi
AU - Garaci, Enrico
PY - 1996/5/3
Y1 - 1996/5/3
N2 - Production of nerve growth factor (NGF) was assessed in cultures of human T and B lymphocytes and macrophages. NGF was constitutively produced by B cells only, which also expressed surface p140(trk-A) and p75(NGFR) molecules and hence efficiently bound and internalized the cytokine. Neutralization of endogenous NGF caused disappearance of Bcl-2 protein and apoptotic death of resting lymphocytes bearing surface IgG or IgA, a population comprising memory cells, while surface IgM/IgD 'virgin' B lymphocytes were not affected. In vivo administration of neutralizing anti-NGF antibodies caused strong reduction in the titer of specific IgG in mice immunized with tetanus toxoid, nitrophenol, or arsonate and reduced numbers of surface IgG or IgA B lymphocytes. Thus, NGF is an autocrine survival factor for memory B lymphocytes.
AB - Production of nerve growth factor (NGF) was assessed in cultures of human T and B lymphocytes and macrophages. NGF was constitutively produced by B cells only, which also expressed surface p140(trk-A) and p75(NGFR) molecules and hence efficiently bound and internalized the cytokine. Neutralization of endogenous NGF caused disappearance of Bcl-2 protein and apoptotic death of resting lymphocytes bearing surface IgG or IgA, a population comprising memory cells, while surface IgM/IgD 'virgin' B lymphocytes were not affected. In vivo administration of neutralizing anti-NGF antibodies caused strong reduction in the titer of specific IgG in mice immunized with tetanus toxoid, nitrophenol, or arsonate and reduced numbers of surface IgG or IgA B lymphocytes. Thus, NGF is an autocrine survival factor for memory B lymphocytes.
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U2 - 10.1016/S0092-8674(00)81113-7
DO - 10.1016/S0092-8674(00)81113-7
M3 - Article
C2 - 8616890
AN - SCOPUS:0029898913
VL - 85
SP - 345
EP - 356
JO - Cell
JF - Cell
SN - 0092-8674
IS - 3
ER -