Nerve growth factor modulates in vitro the expression and release of TGF-β1 by amniotic membrane

Marco Coassin, Alessandro Lambiase, Alessandra Micera, Paola Tirassa, Luigi Aloe, Stefano Bonini

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Purpose: To determinate the basal production of NGF and TGF-β1 by amniotic membrane (AM) and to verify the presence of NGF receptors (trkA NGFR and p75 NTR) in AM. Thereafter, to evaluate in an in vitro model if increasing concentrations of NGF are able to stimulate AM to produce and release TGF-β1. Methods: Immunohistochemistry, ELISA, in situ hybridization and PCR analysis for NGF, TGF-β1, trkA NGFR and p75 NTR were performed to study their presence in AM. Amniotic membranes were stimulated in vitro with increasing concentrations of NGF. After 24 h, the amount of TGF- β1 in the AM tissue and in the culture medium was investigate. Results: AM expressed TGF-β1, NGF, trkA NGFR and p75 NTR mRNAs and proteins and released basal amounts of NGF and TGF-β1 in the medium. Stimulation of AM by addition of NGF induced a significant (P

Original languageEnglish
Pages (from-to)485-491
Number of pages7
JournalGraefe's Archive for Clinical and Experimental Ophthalmology
Volume244
Issue number4
DOIs
Publication statusPublished - Apr 2006

Fingerprint

Amnion
Nerve Growth Factor
Nerve Growth Factor Receptors
In Vitro Techniques
In Situ Hybridization
Culture Media
Enzyme-Linked Immunosorbent Assay
Immunohistochemistry
Polymerase Chain Reaction
Messenger RNA
Membranes

Keywords

  • Amniotic membrane
  • Amniotic membrane transplantation
  • Nerve growth factor
  • Transforming growth factor-β1

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Nerve growth factor modulates in vitro the expression and release of TGF-β1 by amniotic membrane. / Coassin, Marco; Lambiase, Alessandro; Micera, Alessandra; Tirassa, Paola; Aloe, Luigi; Bonini, Stefano.

In: Graefe's Archive for Clinical and Experimental Ophthalmology, Vol. 244, No. 4, 04.2006, p. 485-491.

Research output: Contribution to journalArticle

Coassin, Marco ; Lambiase, Alessandro ; Micera, Alessandra ; Tirassa, Paola ; Aloe, Luigi ; Bonini, Stefano. / Nerve growth factor modulates in vitro the expression and release of TGF-β1 by amniotic membrane. In: Graefe's Archive for Clinical and Experimental Ophthalmology. 2006 ; Vol. 244, No. 4. pp. 485-491.
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AU - Bonini, Stefano

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