Nerve growth factor (NGF) reduces and NGF antibody exacerbates retinal damage induced in rabbit by experimental ocular hypertension

Alessandro Lambiase, Marco Centofanti, Alessandra Micera, Gian Luca Manni, Eleonora Mattei, Alessandra De Gregorio, Giuseppe De Feo, Massimo Gilberto Bucci, Luigi Aloe

Research output: Contribution to journalArticle

65 Citations (Scopus)

Abstract

Background: It has been shown that intravitreal injection of NGF inhibits ganglion cell degeneration after optic nerve transection and ischemic injury. The aim of our study was to investigate the presence of NGF in aqueous humor and its involvement in retinal damage during ocular hypertension. Methods: We used an experimental model of ocular hypertension in rabbit. Before treatment and 4, 10 and 15 days after induction of hypertension, we evaluated histological retinal damage and NGF levels in aqueous humor using an immunoenzymatic assay. Polyclonal anti-NGF antibodies were injected intravitreally into one eye of each rabbit (n = 6), and the animals were killed after 4 days of hypertension. Another group of rabbits (n = 12) was injected retro-ocularly with NGF and killed after 10 or 15 days of treatment for histologic evaluation of the retina. Results: Our data show that experimental ocular hypertension in adult rabbits induces retinal damage and enhances local NGF levels. The highest NGF value was found after 4 days of intraocular hypertension; high levels persisted, though to a lesser extent, for up to 15 days. Histological examination revealed that the number of retinal ganglion cells (RGC) remained unchanged during the first 4 days but decreased at 10 days. These studies also showed that retro-ocular administration of NCF reduced RGC loss, whereas intraocular injection of NGF antibodies, which inhibited the endogenous NGF, exacerbated the retinal insult. Conclusion: These findings demonstrate a protective effect of NGF on RGC damaged by ocular hypertension and prompt further investigations to evaluate a possible therapeutic use of NGF to retard RGC death in humans.

Original languageEnglish
Pages (from-to)780-785
Number of pages6
JournalGraefe's Archive for Clinical and Experimental Ophthalmology
Volume235
Issue number12
Publication statusPublished - Dec 1997

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Ocular Hypertension
Nerve Growth Factor
Rabbits
Antibodies
Retinal Ganglion Cells
Aqueous Humor
Hypertension
Ophthalmic Administration
Intraocular Injections
Optic Nerve Injuries
Intravitreal Injections
Therapeutic Uses
Ganglia
Retina
Cell Death
Theoretical Models

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Nerve growth factor (NGF) reduces and NGF antibody exacerbates retinal damage induced in rabbit by experimental ocular hypertension. / Lambiase, Alessandro; Centofanti, Marco; Micera, Alessandra; Manni, Gian Luca; Mattei, Eleonora; De Gregorio, Alessandra; De Feo, Giuseppe; Bucci, Massimo Gilberto; Aloe, Luigi.

In: Graefe's Archive for Clinical and Experimental Ophthalmology, Vol. 235, No. 12, 12.1997, p. 780-785.

Research output: Contribution to journalArticle

Lambiase, A, Centofanti, M, Micera, A, Manni, GL, Mattei, E, De Gregorio, A, De Feo, G, Bucci, MG & Aloe, L 1997, 'Nerve growth factor (NGF) reduces and NGF antibody exacerbates retinal damage induced in rabbit by experimental ocular hypertension', Graefe's Archive for Clinical and Experimental Ophthalmology, vol. 235, no. 12, pp. 780-785.
Lambiase, Alessandro ; Centofanti, Marco ; Micera, Alessandra ; Manni, Gian Luca ; Mattei, Eleonora ; De Gregorio, Alessandra ; De Feo, Giuseppe ; Bucci, Massimo Gilberto ; Aloe, Luigi. / Nerve growth factor (NGF) reduces and NGF antibody exacerbates retinal damage induced in rabbit by experimental ocular hypertension. In: Graefe's Archive for Clinical and Experimental Ophthalmology. 1997 ; Vol. 235, No. 12. pp. 780-785.
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AU - Mattei, Eleonora

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