Nerve growth factor promotes the differentiation of pituitary mammotroph cells in vitro

C. Missale, F. Boroni, M. Frassine, A. Caruso, P. Spano

Research output: Contribution to journalArticle

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Abstract

It is well known that the differentiation of somatotroph cells is dependent on GRF. The extracellular signals that direct the initial proliferation and differentiation of mammotroph cells during pituitary development, however, have remained elusive. In the present study we first report that somatomammotroph and mammotroph cells present in early postnatal rat pituitary cultures express the receptor for nerve growth factor (NGF) and contain NGF. In addition, we demonstrate that endogenous NGF supports the proliferation and terminal differentiation of mammotroph cells during postnatal in vitro maturation. Early postnatal rat pituitary cells were cultured for 1-12 days, double stained with antibodies to PRL and GH, and analyzed by fluorescence-activated cell sorting. Mature mammotroph cells were generated in culture as shown by 1) the increase in PRL production, 2) the spreading of cells secreting only PRL in relation to those producing both PRL and GH, and 3) the expression of D-2 receptors for dopamine. Exposure of cell cultures to NGF produced a remarkable increase in the rate of appearance of mammotroph cells and an increase in their abundance from 20 ± 0.5% to 44 ± 1%. Deprivation of secreted NGF by means of a specific antibody completely prevented the generation of mature mammotrophs. The fluorescence-activated cell sorting analysis of cells grown in the presence of the antibody to NGF showed, in fact, that although the proportions of somatotrophs and somatomammotrophs were unchanged, that of mammotrophs was much lower than that in untreated cultures. We, thus, conclude that NGF is critical for generation of the mammotroph phenotype during pituitary maturation.

Original languageEnglish
Pages (from-to)1205-1213
Number of pages9
JournalEndocrinology
Volume136
Issue number3
Publication statusPublished - 1995

Fingerprint

Nerve Growth Factor
Somatotrophs
Cell Differentiation
Antibodies
Flow Cytometry
Nerve Growth Factor Receptor
Dopamine Receptors
In Vitro Techniques
Cultured Cells
Cell Culture Techniques
Phenotype

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Missale, C., Boroni, F., Frassine, M., Caruso, A., & Spano, P. (1995). Nerve growth factor promotes the differentiation of pituitary mammotroph cells in vitro. Endocrinology, 136(3), 1205-1213.

Nerve growth factor promotes the differentiation of pituitary mammotroph cells in vitro. / Missale, C.; Boroni, F.; Frassine, M.; Caruso, A.; Spano, P.

In: Endocrinology, Vol. 136, No. 3, 1995, p. 1205-1213.

Research output: Contribution to journalArticle

Missale, C, Boroni, F, Frassine, M, Caruso, A & Spano, P 1995, 'Nerve growth factor promotes the differentiation of pituitary mammotroph cells in vitro', Endocrinology, vol. 136, no. 3, pp. 1205-1213.
Missale C, Boroni F, Frassine M, Caruso A, Spano P. Nerve growth factor promotes the differentiation of pituitary mammotroph cells in vitro. Endocrinology. 1995;136(3):1205-1213.
Missale, C. ; Boroni, F. ; Frassine, M. ; Caruso, A. ; Spano, P. / Nerve growth factor promotes the differentiation of pituitary mammotroph cells in vitro. In: Endocrinology. 1995 ; Vol. 136, No. 3. pp. 1205-1213.
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