Nested polymerase chain reaction for high-sensitivity detection of enteroviral RNA in biological samples

G. M. Severini, L. Mestroni, A. Falaschi, F. Camerini, M. Giacca

Research output: Contribution to journalArticlepeer-review

Abstract

A method based on nested polymerase chain reaction was developed for the detection of enteroviral genomes in biological samples. By taking advantage of the conserved 5' noncoding region of the enteroviral RNA, two sets of primers were utilized, enabling the detection either of a broad range of enteroviruses or of group B coxsackieviruses only. The sensitivity of the method is close to the detection of single molecules of viral RNA in as much as 1 mg of tissue sample. A preliminary study showed the usefulness of this technique for the analysis of endomyocardial biopsy samples from patients with idiopathic dilated cardiomyopathy and myocarditis.

Original languageEnglish
Pages (from-to)1345-1349
Number of pages5
JournalJournal of Clinical Microbiology
Volume31
Issue number5
Publication statusPublished - 1993

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

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