Nested polymerase chain reaction on vaginal smears of tuberculous cervicitis: A case report

Gerardo Ferrara, Maria Cannone, Antonio Guadagnino, Oscar Nappi, Massimo C. Barberis

Research output: Contribution to journalArticle

Abstract

BACKGROUND: Tuberculous cervicitis (TC) is a rare disease the diagnosis of which is based on the microscopic and/or cultural recognition of mycobacteria. In recent years, the polymerase chain reaction (PCR), especially with double-round amplification ('nested' PCR [nPCR]), has been increasingly used for rapid detection of mycobacteria in clinical samples. CASE: The present case is the first example of tuberculosis diagnosed with the aid of nPCR amplification of mycobacterial DNA fragments on smeared and Papanicolaou-stained cytologic material. First detected on vaginal smears, the amplicon IS6110 was subsequently identified also on paraffin-embedded tissue sections. CONCLUSION: The technique described here could also be applied to aspiration cytology smears to give rapid and accurate information on mycobacterial infections.

Original languageEnglish
Pages (from-to)308-312
Number of pages5
JournalActa Cytologica
Volume43
Issue number2
Publication statusPublished - 1999

Keywords

  • Cervicitis
  • Nested polymerase chain reaction
  • Polymerase chain reaction
  • Tuberculous cervicitis
  • Vaginal smears

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology
  • Histology

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