NEU3 sialidase strictly modulates GM3 levels in skeletal myoblasts C2C12 thus favoring their differentiation and protecting them from apoptosis

Luigi Anastasia, Nadia Papini, Francesca Colazzo, Giacomo Palazzolo, Cristina Tringali, Loredana Dileo, Marco Piccoli, Erika Conforti, Clementina Sitzia, Eugenio Monti, Maurilio Sampaolesi, Guido Tettamanti, Bruno Venerando

Research output: Contribution to journalArticle

Abstract

Membrane-bound sialidase NEU3, often referred to as the "ganglioside sialidase," has a critical regulatory function on the sialoglycosphingolipid pattern of the cell membrane, with an anti-apoptotic function, especially in cancer cells. Although other sialidases have been shown to be involved in skeletal muscle differentiation, the role of NEU3 had yet to be disclosed. Herein we report that NEU3 plays a key role in skeletal muscle differentiation by strictly modulating the ganglioside content of adjacent cells, with special regard to GM3. Induced down-regulation of NEU3 in murine C2C12 myoblasts, even when partial, totally inhibits their capability to differentiate by increasing the GM3 level above a critical point, which causes epidermal growth factor receptor inhibition (and ultimately its down-regulation) and an higher responsiveness of myoblasts to the apoptotic stimuli.

Original languageEnglish
Pages (from-to)36265-36271
Number of pages7
JournalJournal of Biological Chemistry
Volume283
Issue number52
DOIs
Publication statusPublished - Dec 26 2008

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

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    Anastasia, L., Papini, N., Colazzo, F., Palazzolo, G., Tringali, C., Dileo, L., Piccoli, M., Conforti, E., Sitzia, C., Monti, E., Sampaolesi, M., Tettamanti, G., & Venerando, B. (2008). NEU3 sialidase strictly modulates GM3 levels in skeletal myoblasts C2C12 thus favoring their differentiation and protecting them from apoptosis. Journal of Biological Chemistry, 283(52), 36265-36271. https://doi.org/10.1074/jbc.M805755200