TY - JOUR
T1 - Neural crest neuroblasts can colonise aganglionic and ganglionic gut in vivo
AU - Martucciello, GLuseppe
AU - Brizzolara, A.
AU - Favre, A.
AU - Lombardi, L.
AU - Bocciardi, R.
AU - Sanguineti, M.
AU - Pini Prato, A.
AU - Jasonni, V.
PY - 2007/2
Y1 - 2007/2
N2 - Introduction: Neural crest (NC) cells differentiate in vitro into neuroblasts, precursors of the enteric nervous system (ENS), when stimulated by specific agents. We developed a study aimed at establishing whether NC-derived neuroblasts can survive and colonise in vivo when injected into a recipient mouse gut. Materials and Methods: The neuroblast precursors of the ENS were obtained from the vagal portion of the neural tubes of 296 CD-1 and Gtrosa26 mouse embryos. The embryonic cells of Gtrosa26 mice are identifiable through beta-galactosidase activity which allows recognition by blue staining. The host used in this study was the Dom/+ mouse, an animal model for Hirschsprung's disease (aganglionic megacolon). Dom/+ mouse pups (n = 43) received NC-derived cells inoculated into the seromuscular layer of the gut (33/43) or directly into the peritoneal abdominal cavity (10/ 43). Results: All Dom/+ mice survived the procedure and were sacrificed after 7 or 14 days. Histochemical staining detected implanted cells in all mice. These showed specific myenteric colonisation into the aganglionic and ganglionic gut. Conclusion: The striking result of this study was the specific tropism of the injected NC-derived cells to target sites under the action of unknown chemotactic agents. This experimental procedure might represent a possible treatment option for specific forms of human ENS anomaly such as total intestinal aganglionosis.
AB - Introduction: Neural crest (NC) cells differentiate in vitro into neuroblasts, precursors of the enteric nervous system (ENS), when stimulated by specific agents. We developed a study aimed at establishing whether NC-derived neuroblasts can survive and colonise in vivo when injected into a recipient mouse gut. Materials and Methods: The neuroblast precursors of the ENS were obtained from the vagal portion of the neural tubes of 296 CD-1 and Gtrosa26 mouse embryos. The embryonic cells of Gtrosa26 mice are identifiable through beta-galactosidase activity which allows recognition by blue staining. The host used in this study was the Dom/+ mouse, an animal model for Hirschsprung's disease (aganglionic megacolon). Dom/+ mouse pups (n = 43) received NC-derived cells inoculated into the seromuscular layer of the gut (33/43) or directly into the peritoneal abdominal cavity (10/ 43). Results: All Dom/+ mice survived the procedure and were sacrificed after 7 or 14 days. Histochemical staining detected implanted cells in all mice. These showed specific myenteric colonisation into the aganglionic and ganglionic gut. Conclusion: The striking result of this study was the specific tropism of the injected NC-derived cells to target sites under the action of unknown chemotactic agents. This experimental procedure might represent a possible treatment option for specific forms of human ENS anomaly such as total intestinal aganglionosis.
KW - Animal model
KW - Enteric nervous system (ENS)
KW - Hirschsprung's disease (HSCR)
KW - Neural crest (NC)
KW - Stem cells
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U2 - 10.1055/s-2007-964952
DO - 10.1055/s-2007-964952
M3 - Article
C2 - 17407019
AN - SCOPUS:34249018510
VL - 17
SP - 34
EP - 40
JO - European Journal of Pediatric Surgery
JF - European Journal of Pediatric Surgery
SN - 0939-7248
IS - 1
ER -