Neural progenitor cells orchestrate microglia migration and positioning into the developing cortex

Benedetta Arnò, Francesca Grassivaro, Chiara Rossi, Andrea Bergamaschi, Valentina Castiglioni, Roberto Furlan, Melanie Greter, Rebecca Favaro, Giancarlo Comi, Burkhard Becher, Gianvito Martino, Luca Muzio

Research output: Contribution to journalArticle

84 Citations (Scopus)

Abstract

Microglia are observed in the early developing forebrain and contribute to the regulation of neurogenesis through still unravelled mechanisms. In the developing cerebral cortex, microglia cluster in the ventricular/subventricular zone (VZ/SVZ), a region containing Cxcl12-expressing basal progenitors (BPs). Here we show that the ablation of BP as well as genetic loss of Cxcl12 affect microglia recruitment into the SVZ. Ectopic Cxcl12 expression or pharmacological blockage of CxcR4 further supports that Cxcl12/CxcR4 signalling is involved in microglial recruitment during cortical development. Furthermore, we found that cell death in the developing forebrain triggers microglial proliferation and that this is mediated by the release of macrophage migration inhibitory factor (MIF). Finally, we show that the depletion of microglia in mice lacking receptor for colony-stimulating factor-1 (Csf-1R) reduces BPs into the cerebral cortex.

Original languageEnglish
Article number5611
JournalNature Communications
Volume5
DOIs
Publication statusPublished - 2014

Fingerprint

cerebral cortex
cortexes
Microglia
positioning
Stem Cells
macrophages
Prosencephalon
cells
death
Cerebral Cortex
ablation
mice
depletion
actuators
Macrophage Migration-Inhibitory Factors
Macrophage Colony-Stimulating Factor
Lateral Ventricles
Neurogenesis
Cell death
Ablation

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Chemistry(all)
  • Physics and Astronomy(all)
  • Medicine(all)

Cite this

Neural progenitor cells orchestrate microglia migration and positioning into the developing cortex. / Arnò, Benedetta; Grassivaro, Francesca; Rossi, Chiara; Bergamaschi, Andrea; Castiglioni, Valentina; Furlan, Roberto; Greter, Melanie; Favaro, Rebecca; Comi, Giancarlo; Becher, Burkhard; Martino, Gianvito; Muzio, Luca.

In: Nature Communications, Vol. 5, 5611, 2014.

Research output: Contribution to journalArticle

Arnò, Benedetta ; Grassivaro, Francesca ; Rossi, Chiara ; Bergamaschi, Andrea ; Castiglioni, Valentina ; Furlan, Roberto ; Greter, Melanie ; Favaro, Rebecca ; Comi, Giancarlo ; Becher, Burkhard ; Martino, Gianvito ; Muzio, Luca. / Neural progenitor cells orchestrate microglia migration and positioning into the developing cortex. In: Nature Communications. 2014 ; Vol. 5.
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AU - Furlan, Roberto

AU - Greter, Melanie

AU - Favaro, Rebecca

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