Neuroanatomical localization and quantification of amyloid precursor protein mRNA by in situ hybridization in the brains of normal, aneuploid, and lesioned mice

C. Bendotti, G. L. Forloni, R. A. Morgan, B. F. O'Hara, M. L. Oster-Granite, R. H. Reeves, J. D. Gearhart, J. T. Coyle

Research output: Contribution to journalArticle

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Abstract

Amyloid precursor protein mRNA was localized in frozen sections from normal and experimentally lesioned adult mouse brain and from normal and aneuploid fetal mouse brain by in situ hybridization with a 35S-labeled mouse cDNA probe. The highest levels of hybridization in adult brain were associated with neurons, primarily in telencephalic structures. The dense labeling associated with hippocampal pyramidal cells was reduced significantly when the cells were eliminated by injection of the neurotoxin ibotenic acid but was not affected when electrolytic lesions were placed in the medial septum. Since the gene encoding amyloid precursor protein has been localized to mouse chromosome 16, we also examined the expression of this gene in the brains of mouse embryos with trisomy 16 and trisomy 19 at 15 days of gestation. RNA gel blot analysis and in situ hybridization showed a marked increase in amyloid precursor protein mRNA in the trisomy 16 mouse head and brain when compared with euploid littermates or with trisomy 19 mice.

Original languageEnglish
Pages (from-to)3628-3632
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume85
Issue number10
Publication statusPublished - 1988

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Amyloid beta-Protein Precursor
Aneuploidy
In Situ Hybridization
Messenger RNA
Brain
Trisomy
Ibotenic Acid
Chromosomes, Human, Pair 16
Telencephalon
Pyramidal Cells
Neurotoxins
Frozen Sections
Embryonic Structures
Complementary DNA
Gels
Head
RNA
Gene Expression
Neurons
Pregnancy

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Neuroanatomical localization and quantification of amyloid precursor protein mRNA by in situ hybridization in the brains of normal, aneuploid, and lesioned mice. / Bendotti, C.; Forloni, G. L.; Morgan, R. A.; O'Hara, B. F.; Oster-Granite, M. L.; Reeves, R. H.; Gearhart, J. D.; Coyle, J. T.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 85, No. 10, 1988, p. 3628-3632.

Research output: Contribution to journalArticle

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AU - O'Hara, B. F.

AU - Oster-Granite, M. L.

AU - Reeves, R. H.

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